Rhythmic 24-hour variations of frequently used clinical biochemical parameters in healthy young males--the Bispebjerg study of diurnal variations.

Author(s): Sennels HP, Jørgensen HL, Goetze JP, Fahrenkrug J

Publication: Scand J Clin Lab Invest, 2012, Vol. 72, Page 287-95

PubMed ID: 22397709 PubMed Review Paper? No

Purpose of Paper

The purpose of this paper was to determine the effects of time of collection on the levels of 15 clinical chemistry analytes in plasma and serum.

Conclusion of Paper

A normal diurnal melatonin rhythm was used to validate that the diurnal activity and nocturnal sleep patterns of all volunteers were normal. Although the concentrations of most of the analytes were within normal ranges, regardless of biospecimen collection time, 8 of the 14 analytes did show significant diurnal rhythms over the 24 h collection period. Uric acid levels peaked in the morning, potassium, bilirubin, and sodium peaked midday, aspartate amino transferase (AST), creatinine kinase, and lactate dehydrogenase (LDH) levels peaked in the afternoon, and urea nitrogen levels peaked around midnight.

Studies

Study Purpose

The purpose of this study was to determine the effects of time of collection on the levels of 15 clinical chemistry analytes in plasma and serum. All analytes, except P-Amylase and melatonin, were measured in fresh plasma collected in lithium heparin tubes. Specimens intended for P-Amylase and melatonin analysis were collected into serum clot activator tubes. Serum was frozen at -80 degrees C prior to analysis.

Summary of Findings:

A normal diurnal melatonin rhythm was used to confirm that the diurnal activity and nocturnal sleep patterns of all volunteers were normal. Although the concentrations of most of the analytes were within normal ranges, regardless of biospecimen collection time, 8 of the 14 analytes did show significant diurnal rhythms over the 24 h collection period. Uric acid levels peaked in the morning, potassium, bilirubin, and sodium peaked midday, AST, creatinine kinase, and LDH levels peaked in the afternoon, and urea nitrogen levels peaked around midnight. Magnesium, creatinine, estimated glomerular filtration rate (EGFR) by Modification of diet in renal Disease formula, alanine aminotransferase (ALT), gamma glutamyltransferase (GGT), and P-amylase did not show significantly different levels based on collection time.

Biospecimens

Preservative Types

None (Fresh)

Diagnoses:

Normal

Platform:

Analyte	Technology Platform
Small molecule	Clinical chemistry/auto analyzer
Electrolyte/Metal	Clinical chemistry/auto analyzer
Protein	Clinical chemistry/auto analyzer
Small molecule	Radioimmunoassay

Pre-analytical Factors:

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