Storage-induced increase in biomarkers of oxidative stress and inflammation in red blood cell components.

Author(s): Kücükakin B, Kocak V, Lykkesfeldt J, Nielsen HJ, Magnussen K, Rosenberg J, Gögenur I

Publication: Scand J Clin Lab Invest, 2011, Vol. 71, Page 299-303

PubMed ID: 21385017 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of refrigerated storage on markers of inflammatory and oxidative stress in red blood cells. Supernatants were frozen at -80 degrees C before analysis.

   Summary of Findings:

   MDA levels decreased in RBC stored for 3 days compared to RBCs stored for 1 day (p=0.008) but were significantly higher in specimens stored for 35 days than those stored for 1 day (p<0.001). DHA and YKL-40 levels increased progressively during refrigerated storage (p<0.001). IL6 and ASC levels in RBCs increased with storage, peaking at day 21 and 14, respectively, and then decreasing slightly. AT levels in RBCs were not significantly affected by up to 35 days of refrigerated storage.

   Biospecimens

   • Cell - Red Blood Cells

   Preservative Types

   • Other Preservative

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Small molecule	HPLC
   Protein	ELISA
   Glycoprotein	Radioimmunoassay

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage duration	1 day 3 days 7 days 14 days 21 days 28 days 35 days

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