Effect of freeze-thaw cycles on serum measurements of AFP, CEA, CA125 and CA19-9.

Author(s): Gao YC, Yuan ZB, Yang YD, Lu HK

Publication: Scand J Clin Lab Invest, 2007, Vol. 67, Page 741-7

PubMed ID: 17852813 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine if freeze-thaw cycling (the number and duration of interim storage) can alter the immunoreactivity of tumor markers in serum specimens.

   Summary of Findings:

   Serum specimens subjected to 1 to 3 freeze-thaw cycles did not differ from baseline or one another for AFP, CEA, or CA125 levels, although these tumor markers were significantly lower after 4 freeze-thaw cycles, which corresponded to a shift in storage duration from 2 hours to 10 months. CA19-9 levels decreased progressively after just 1 freeze-thaw cycle.

   Biospecimens

   • Bodily Fluid - Serum

   Preservative Types

   • Frozen

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Protein	Immunoassay

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Freeze/thaw cycling	0 cycles 1 cycle 2 cycles 3 cycles 4 cycles 5 cycles

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