Stability of YKL-40 concentration in blood samples.
Author(s): Høgdall EV, Johansen JS, Kjaer SK, Price PA, Blaakjaer J, Høgdall CK
Publication: Scand J Clin Lab Invest, 2000, Vol. 60, Page 247-51
PubMed ID: 10943594 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
The purpose of this study was to determine the effects of delayed centrifugation, storage, and freeze-thaw cycling on the stability of YKL-40 in serum and plasma. After separation, serum and plasma were stored at -20 degrees C until analysis.
Summary of Findings:
Compared with a clotting time of 1 hour, YKL-40 was significantly elevated in serum specimens when blood was left at room temperature (24 degrees C) for more than 3 hours prior to centrifugation. In EDTA plasma, significant elevations in YKL-40 were only seen when blood was left at room temperature for over 8 hours prior to centrifugation. A minor but significant decrease (p=0.05) in EDTA plasma YKL-40 was also seen after blood was left at room temperature for 3 hours prior to centrifugation. Storage of blood at 4 degrees C for 24 hours and 72 hours prior to centrifugation had no impact on YKL-40 levels in serum or EDTA plasma, respectively. In general, EDTA plasma had lower levels of YKL-40 than serum or heparin plasma, and heparin plasma had lower levels of YKL-40 than serum. Up to 7 freeze-thaw cycles had no effect on YKL-40 stability in serum.
Analyte Technology Platform Glycoprotein ELISA
Classification Pre-analytical Factor Value(s) Biospecimen Acquisition Anticoagulant EDTA
Storage Storage temperature 24 degrees C
4 degrees C
Storage Storage duration 1 h
Storage Freeze/thaw cycling 1 cycle
Preaquisition Patient gender Female
Biospecimen Aliquots and Components Blood and blood products Plasma
Biospecimen Aliquots and Components Centrifugation Centrifugation delays investigated