NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

Stability of YKL-40 concentration in blood samples.

Author(s): Høgdall EV, Johansen JS, Kjaer SK, Price PA, Blaakjaer J, Høgdall CK

Publication: Scand J Clin Lab Invest, 2000, Vol. 60, Page 247-51

PubMed ID: 10943594 PubMed Review Paper? No

Purpose of Paper

The purpose of this paper was to determine the effects of delayed centrifugation and storage temperature on the stability of human cartilage glycoprotein-39 (YKL-40) in serum and plasma.

Conclusion of Paper

When blood was stored at room temperature, but not 4 degrees C, for more than 3 and 8 h, YKL-40 was significantly elevated in serum and EDTA plasma, respectively. In general, EDTA plasma had lower levels of YKL-40 than serum or heparin plasma, and heparin plasma had lower levels of YKL-40 than serum. Up to 7 freeze-thaw cycles had no effect on YKL-40 stability in serum.

Studies

  1. Study Purpose

    The purpose of this study was to determine the effects of delayed centrifugation, storage, and freeze-thaw cycling on the stability of YKL-40 in serum and plasma. After separation, serum and plasma were stored at -20 degrees C until analysis.

    Summary of Findings:

    Compared with a clotting time of 1 hour, YKL-40 was significantly elevated in serum specimens when blood was left at room temperature (24 degrees C) for more than 3 hours prior to centrifugation. In EDTA plasma, significant elevations in YKL-40 were only seen when blood was left at room temperature for over 8 hours prior to centrifugation. A minor but significant decrease (p=0.05) in EDTA plasma YKL-40 was also seen after blood was left at room temperature for 3 hours prior to centrifugation. Storage of blood at 4 degrees C for 24 hours and 72 hours prior to centrifugation had no impact on YKL-40 levels in serum or EDTA plasma, respectively. In general, EDTA plasma had lower levels of YKL-40 than serum or heparin plasma, and heparin plasma had lower levels of YKL-40 than serum. Up to 7 freeze-thaw cycles had no effect on YKL-40 stability in serum.

    Biospecimens
    Preservative Types
    • Frozen
    Diagnoses:
    • Normal
    Platform:
    AnalyteTechnology Platform
    Glycoprotein ELISA
    Pre-analytical Factors:
    ClassificationPre-analytical FactorValue(s)
    Biospecimen Acquisition Anticoagulant EDTA
    Heparin
    None
    Storage Storage temperature 24 degrees C
    4 degrees C
    Storage Storage duration 1 h
    3 h
    8 h
    24 h
    72 h
    Storage Freeze/thaw cycling 1 cycle
    2 cycles
    3 cycles
    4 cycles
    5 cycles
    6 cycles
    7 cycles
    Preaquisition Patient gender Female
    Biospecimen Aliquots and Components Blood and blood products Plasma
    Serum
    Biospecimen Aliquots and Components Centrifugation Centrifugation delays investigated

You Recently Viewed  

comments powered by Disqus

News and Announcements

  • BRD Visitor Survey is Now Open!

  • NEW Expert-Vetted BEBP Now Available!

  • Most Popular SOPs in 2019

  • More...