Stability of YKL-40 concentration in blood samples.

Author(s): Høgdall EV, Johansen JS, Kjaer SK, Price PA, Blaakjaer J, Høgdall CK

Publication: Scand J Clin Lab Invest, 2000, Vol. 60, Page 247-51

PubMed ID: 10943594 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of delayed centrifugation, storage, and freeze-thaw cycling on the stability of YKL-40 in serum and plasma. After separation, serum and plasma were stored at -20 degrees C until analysis.

   Summary of Findings:

   Compared with a clotting time of 1 hour, YKL-40 was significantly elevated in serum specimens when blood was left at room temperature (24 degrees C) for more than 3 hours prior to centrifugation. In EDTA plasma, significant elevations in YKL-40 were only seen when blood was left at room temperature for over 8 hours prior to centrifugation. A minor but significant decrease (p=0.05) in EDTA plasma YKL-40 was also seen after blood was left at room temperature for 3 hours prior to centrifugation. Storage of blood at 4 degrees C for 24 hours and 72 hours prior to centrifugation had no impact on YKL-40 levels in serum or EDTA plasma, respectively. In general, EDTA plasma had lower levels of YKL-40 than serum or heparin plasma, and heparin plasma had lower levels of YKL-40 than serum. Up to 7 freeze-thaw cycles had no effect on YKL-40 stability in serum.

   Biospecimens

   • Bodily Fluid - Serum
   • Bodily Fluid - Plasma
   • Bodily Fluid - Blood

   Preservative Types

   • Frozen

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Glycoprotein	ELISA

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Anticoagulant	EDTA Heparin None
   Storage	Storage temperature	24 degrees C 4 degrees C
   Storage	Storage duration	1 h 3 h 8 h 24 h 72 h
   Storage	Freeze/thaw cycling	1 cycle 2 cycles 3 cycles 4 cycles 5 cycles 6 cycles 7 cycles
   Preaquisition	Patient gender	Female
   Biospecimen Aliquots and Components	Blood and blood products	Plasma Serum
   Biospecimen Aliquots and Components	Centrifugation	Centrifugation delays investigated

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