The effect of the order of filling tubes after venipuncture on serum potassium, total protein, and aspartate and alanine aminotransferases.

Author(s): Leppänen E, Gräsbeck R

Publication: Scand J Clin Lab Invest, 1986, Vol. 46, Page 189-91

PubMed ID: 3715371 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of aliquot sequential collection (from a single venipuncture) on serum K, total protein, ASAT, and ALAT measurements. The first and last tubes collected were compared, with the last tube being anywhere from the 2nd tube to the 11th tube. Tourniquets were not used. The authors do not specify whether serum was analyzed immediately or stored.

   Summary of Findings:

   Serum protein, ASAT, and ALAT concentrations were not significantly different between the first 10 mL blood specimen drawn and the last 10 mL blood specimen drawn from the same venipuncture. However, serum K was significantly higher in the first 10 mL aliquot than the last 10 mL aliquot (+0.09 mmol/L, p<0.001). Further, serum K was significantly higher in the first 1 mL blood aliquot compared to the 2nd aliquot consisting of 10 mL (+0.08 mmol/L, p<0.001). Importantly, these differences were similar to the precision of the equipment used for the K measurements bringing clinical significance into question.

   Biospecimens

   • Bodily Fluid - Serum
   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Electrolyte/Metal	Flame emission photometry
   Protein	Clinical chemistry/auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Aliquots and Components	Aliquot sequential collection	1st collection Not first collection
   Biospecimen Aliquots and Components	Aliquot size/volume	1 mL 10 mL

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