Systematic analysis of microRNA expression of RNA extracted from fresh frozen and formalin-fixed paraffin-embedded samples.
Author(s): Xi Y, Nakajima G, Gavin E, Morris CG, Kudo K, Hayashi K, Ju J
Publication: RNA, 2007, Vol. 13, Page 1668-74
PubMed ID: 17698639 PubMed Review Paper? No
Purpose of Paper
The purpose of this paper was to determine the effects of storage on the quantification of 5s ribosomal RNA (rRNA) and microRNA 181b (miR-181b) in formalin-fixed paraffin-embedded (FFPE) colorectal carcinoma specimens.
Conclusion of Paper
There was no effect of storage duration on the quantification of 5s rRNA and miR-181b in FFPE specimens by low density array. RNA of quality suitable for array analysis was isolated from specimens that were up to 10 y old.
Studies
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Study Purpose
The purpose of this study was to determine the effects of storage on the quantification of 5s rRNA and miR-181b in FFPE colorectal carcinoma specimens.
Summary of Findings:
There was no effect of storage duration on the quantification of 5s rRNA and miR-181b in FFPE specimens by low density array. RNA of quality suitable for array analysis was isolated from specimens that were up to 10 y old.
Biospecimens
Preservative Types
- Formalin
Diagnoses:
- Neoplastic - Carcinoma
Platform:
Analyte Technology Platform RNA Real-time qRT-PCR RNA Low density array Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Storage duration 5 y
6 y
7 y
8 y
9 y
10 y
Low density array Specific Targeted nucleic acid 5s rRNA
miR-181b