Thyroid fine-needle biopsy: aspiration versus capillary.
Author(s): Buzdugă CM, Găleşanu C, Vulpoi C, Preda C, Ungureanu MC, Ciobanu D, Azoicăi D, Mogoş V
Publication: Rev Med Chir Soc Med Nat Iasi, 2015, Vol. 119, Page 45-50
PubMed ID: 25970941 PubMed Review Paper? No
Purpose of Paper
This paper compared markers of cytological quality, sensitivity and specificity in thyroid specimens procured by fine needle aspiration (FNA) or fine needle non-aspiration (FNNA).
Conclusion of Paper
The amount of blood contamination, degree of cellular degeneration, amount of cellular trauma, preservation of appropriate architecture, amount of cellular material, and overall specimen quality were found to be comparable in FNA and FNNA specimens. Diagnostic sensitivity and specificity were also comparable between FNA and FNNA specimens.
Studies
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Study Purpose
This study compared the scores for amount of blood contamination, degree of cellular degeneration, amount of cellular trauma, preservation of appropriate architecture, amount of cellular material, and overall specimen quality as well as the diagnostic sensitivity and specificity among thyroid FNA and FNNA specimens. Specimens were procured by either FNA in 34 patients or by FNNA in 275 patients from thyroid lesions and case-matched formalin-fixed, paraffin-embedded (FFPE) surgical resected specimens. FNA and FNNA biopsies were May-Grünwald-Giemsa stained. Blood contamination was graded as 0 if contamination was severe enough to compromise diagnosis; 1 if contamination was moderate but diagnosis was still possible; and 2 if contamination was minimal and diagnosis was not adversely affected. The amount of cellular material was scored as 0 if diagnosis was not possible, 1 if material was sufficient for diagnosis, and 3 if material was abundant that allowed for easy diagnosis. The degree of cellular degeneration, degree of cellular trauma and preservation of appropriate architecture were scored as 0 if diagnosis was not possible, 1 if diagnosis was possible or easy (degree of cellular trauma only), and 2 if diagnosis was easy or obvious. A cumulative smear quality score of 0-2 was graded as C (unsuitable for diagnosis), a score of 3-5 was graded as B (suitable for cytological diagnosis), and sore of 6-8 was graded as A (excellent for diagnosis).
Summary of Findings:
The percentage of specimens with scores for amount of blood contamination, degree of cellular degeneration, the amount of cellular trauma, preservation of appropriate architecture, and amount of cellular material were comparable between FNA and FNNA thyroid specimens. The collective overall smear quality score also displayed comparable distributions in thyroid specimens obtained by FNA and FNNA. Comparable diagnostic sensitivity and specificity were observed for FNA (71.43% and 91.67%, respectively) and FNNA (75% and 93.75%, respectively) thyroid specimens.
Biospecimens
Preservative Types
- Formalin
- Other Preservative
Diagnoses:
- Neoplastic - Carcinoma
Platform:
Analyte Technology Platform Morphology Light microscopy Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Acquisition Method of cell acquisition Fine needle aspiration biopsy
Fine needle capillary (non-aspiration) biopsy