Cancer Diagnosis Program | Biorepositories and Biospecimen Research Branch

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Impact of needle gauge on characterization of endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) histology samples.

Author(s): Jeyabalan A, Shelley-Fraser G, Medford AR

Publication: Respirology, 2014, Vol. 19, Page 735-9

PubMed ID: 24697875 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   This study compared the diagnostic performance of 21- G and 22-G EBUS-TBNA. EBUS-TBNA specimens were obtained from 303 patients (175 with 21-G needle and 128 with 22-G needle) with unexplained mediastinal lymphadenopathy. For each location, 2 or more passes were obtained, fixed in formalin, and H&E and immunohistochemically stained. Staining was evaluated by lung histopathologists who were blinded to needle gauge. Final diagnosis of NSCLC, small cell lung cancer, metastatic cancer, lymphoma, sarcoidosis, tuberculosis, anthracosis, cyst, reactive nodes, or foamy macrophages was based on 12 months of follow-up.

   Summary of Findings:

   EBUS-TBNA specimens obtained with 21-G and 22-G needles had comparable sensitivity (93.9% and 92.1%, respectively), negative predictive value (92.7% and 89.7%, respectively), and accuracy (96.6% and 95.3%, respectively) for malignant disease.  However, 21-G needles were better than 22-G needles for characterization of benign (83% versus 60%, P<0.01) and NCSLC specimens (88% versus 65%, P<0.01).

   Biospecimens

   • Tissue - Lymph Node
   • Tissue - Lung

   Preservative Types

   • Formalin

   Diagnoses:

   • Neoplastic - Lymphoma
   • Other diagnoses
   • Neoplastic - Benign
   • Neoplastic - Carcinoma

   Platform:

   Analyte	Technology Platform
   Protein	Immunohistochemistry
   Morphology	H-and-E microscopy

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Needle gauge	22-G needle 21-G needle

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