Effects of syringe material, sample storage time, and temperature on blood gases and oxygen saturation in arterialized human blood samples.
Author(s): Knowles TP, Mullin RA, Hunter JA, Douce FH
Publication: Respir Care, 2006, Vol. 51, Page 732-6
PubMed ID: 16800906 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
-
Study Purpose
The purpose of this study was to determine the effects of storage duration, temperature and container on the pH, oxygen saturation, PO2 and PCO2 in blood. Citrate-phosphate-dextrose blood was arterialized with oxygen and carbon dioxide at 37 degrees C and sampled 15 times for each time-point and temperature with plastic syringes containing dry lithium heparin and glass syringes containing dry sodium and lithium heparin.
Summary of Findings:
Compared to blood collected in plastic syringes and analyzed immediately, the PO2 increased by 13.7 and 11.9 mm mercury (Hg) in blood stored in plastic syringes at 0-4 and 22 degrees C, respectively, but the PO2 was unaffected by storage in glass syringes at either temperature. Storage of blood in glass syringes at 0-4 degrees C resulted in a clinically irrelevant decrease in PCO2, but PCO2 was unaffected by storage in plastic syringes at either temperature or in glass syringes at 22 degrees C. pH and oxygen saturation were not affected by storage in either container at 0-4 or 22 degrees C.
Biospecimens
Preservative Types
- None (Fresh)
Diagnoses:
- Not specified
Platform:
Analyte Technology Platform Gas Clinical chemistry/auto analyzer Small molecule pH Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Storage duration 0 min
30 min
Storage Storage temperature 0-4 degrees C
22 degrees C
Storage Type of storage container Plastic syringe
Glass syringe
Biospecimen Acquisition Anticoagulant Lithium heparin
Sodium heparin