Proteome profiling reveals gender differences in the composition of human serum.
Author(s): Miike K, Aoki M, Yamashita R, Takegawa Y, Saya H, Miike T, Yamamura K
Publication: Proteomics, 2010, Vol. 10, Page 2678-91
PubMed ID: 20480504 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to determine the effects of gender on the serum proteome. After separation, the serum from 12 males and 12 females was transferred in non-adsorption tubes and supplemented with protease inhibitors and phosphatase inhibitors and pooled to make 2 male and 2 female serum pools. Peptide identification was completed by MALDI-TOF-TOF MS with isobaric tags for relative and absolute quantification (iTRAQ) analysis.
Summary of Findings:
Overall protein levels were similar in males and females, however, 2D difference gel electrophoresis (DIGE) analysis of protein fractions showed gender specific profiles. 1513 proteins were found to be expressed at similar levels in the two serum specimens from males. Of these, 117 which tended to be associated with breast cancer or arthritis were expressed at more than 1.5 fold higher levels in females than males. 65 proteins were expressed at more than 1.5 fold higher levels in males than females and were generally associated with cascades involving males hormones. 1331 proteins were expressed at similar levels in the males and females and were generally associated with housekeeping functions. 2551 proteins showed variability in expression between serum pools from patients of the same gender. .
Biospecimens
Preservative Types
- None (Fresh)
Diagnoses:
- Not specified
Platform:
Analyte Technology Platform Protein MALDI-TOF MS Protein LC-MS or LC-MS/MS Protein 1D/2D gels Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Preaquisition Patient gender Female
Male