Immunoassay and antibody microarray analysis of the HUPO Plasma Proteome Project reference specimens: systematic variation between sample types and calibration of mass spectrometry data.
Author(s): Haab BB, Geierstanger BH, Michailidis G, Vitzthum F, Forrester S, Okon R, Saviranta P, Brinker A, Sorette M, Perlee L, Suresh S, Drwal G, Adkins JN, Omenn GS
Publication: Proteomics, 2005, Vol. 5, Page 3278-91
PubMed ID: 16038022 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to determine the effects of usage and type of anticoagulant on plasma and serum protein detection by immunoassay and antibody microarray analysis. In addition, comparisons of antibody-based measurements with MS data were made to evaluate the relationship between analyte concentration and the likelihood of protein identification by MS.
Summary of Findings:
General biases in protein concentrations were observed between serum and citrate-, EDTA-, and heparin-plasma. Generally, citrate-plasma had the lowest protein abundances, and serum and EDTA-plasma had the highest protein abundances, but there were many exceptions to these trends. Pooling data from 18 different laboratories revealed a clear dependence of the number of peptides identified and the number of laboratories identifying that particular protein by MS analysis on the particular protein concentration.
Biospecimens
Preservative Types
- Frozen
Diagnoses:
- Not specified
Platform:
Analyte Technology Platform Protein Immunoassay Protein MS/MS Protein Antibody microarray Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Acquisition Anticoagulant Lithium heparin
None
Potassium EDTA
Sodium citrate
Biospecimen Aliquots and Components Blood and blood products Plasma
Serum
Preaquisition Biomarker level Higher analyte concentration
Lower analyte concentration