Optimized preembedding method improves the histologic yield of prostatic core needle biopsies.

Author(s): Rogatsch H, Mairinger T, Horninger W, Gschwendtner A, Bartsch G, Mikuz G

Publication: Prostate, 2000, Vol. 42, Page 124-9

PubMed ID: 10617869 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of stretching and orienting core needle biopsy specimens from a prostatectomy between two meshes, in tissue cassettes, prior to fixation, and the number of cores per tissue block on histologic yield per section of prostate needle biopsy specimens.

   Summary of Findings:

   Stretching and orienting prostate needle biopsy specimens between two nylon meshes, in tissue cassettes, prior to fixation resulted in generally straight cores which were easily aligned in one plane. Conventional processing including fixation while free-floating in formalin resulted in curved cores which were not easily aligned in one plane. Consequently, lower percentages of tissue amount per section level were observed, and twice as many sections were required for complete workup of conventionally processed specimens compared to those that underwent the optimized preembedding method. For paraffin blocks containing three biopsies each, rather than one, 43% and 56% more sections were required for complete workup of specimens that underwent optimized preembedding and conventional processing, respectively.

   Biospecimens

   • Tissue - Prostate

   Preservative Types

   • Formalin

   Diagnoses:

   • Neoplastic - Carcinoma

   Platform:

   Analyte	Technology Platform
   Morphology	Light microscopy

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Type of collection container/solution	Stretched between nylon meshes in tissue cassette Free floating
   Biospecimen Aliquots and Components	Aliquot size/volume	1 biopsy per paraffin block 3 biopsies per paraffin block

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