Salivary inflammatory mediator profiling and correlation to clinical disease markers in asthma.
Author(s): Little FF, Delgado DM, Wexler PJ, Oppenheim FG, Mitchell P, Feldman JA, Walt DR, Peng RD, Matsui EC
Publication: PLoS One, 2014, Vol. 9, Page e84449
PubMed ID: 24409298 PubMed Review Paper? No
Suggested by: Eva J. Helmerhorst, Boston University
Purpose of Paper
The purpose of this paper was to determine the effects of specimen type and patient age, gender and race on markers of asthma control in saliva and nasal lavage specimens.
Conclusion of Paper
Similar principle components were identified in pediatric and adult patients, with the first component reflecting degree of inflammation, and the second reflecting the difference between eosinophil-related markers and myeloid innate immunity markers. Adjustment of the data for age, patient gender, or health characteristics did not affect associations of the principle components with markers of asthma control. Asthma control marker levels in nasal lavage and saliva specimens were not significantly correlated. Further, the ability to obtain a nasal lavage specimen with >20,000 non-squamous cells/mL was not affected by patient age, race, or gender.
Studies
-
Study Purpose
The purpose of this study was to determine the effects of collecting saliva versus nasal lavage and to determine the effects of patient age, gender and race on 10 markers of asthma control using custom Luminex assays. 58 pediatric and 122 adult patients with asthma chewed paraffin wax, and saliva was collected every 30 seconds until 6-10 mL was obtained. Saliva was stored on ice during collection, centrifuged at 13000 g for 20 min and frozen at -80°C. Nasal lavage specimens were also collected from 80 adults.
Summary of Findings:
Similar principle components were identified in pediatric and adult patients, with the first component reflecting degree of inflammation, and the second reflecting the difference between eosinophil-related markers and myeloid innate immunity markers. Adjustment of the data for age, patient gender, or health characteristics/systemic disease did not affect associations of the principle components with markers of asthma control. Asthma control marker levels in nasal lavage and saliva specimens were not significantly correlated. Further, the ability to obtain a nasal lavage specimen with >20,000 non-squamous cells/mL was not affected by patient age, race, or gender.
Biospecimens
Preservative Types
- Frozen
Diagnoses:
- Asthma
- Obesity
- Diabetes Type 2
Platform:
Analyte Technology Platform Protein Immunoassay Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Preaquisition Patient gender Female
Male
Preaquisition Patient age 5-17 years
18-55 years
Immunoassay Specific Targeted peptide/protein 1/CCL11
RANTES/CCL5
IL-5
IL-6
MIP-1b/CCL4
VEGF
IL-8/CXCL8
IL-1b
MCP-1/CCL2
IP-10/CXCL10
Preaquisition Diagnosis/ patient condition Well controlled asthma
Poorly controlled asthma
Uncontrolled asthma
Obesity
Hypotension
Reflux
Diabetes
Autoimmune disease
No additional disease
Preaquisition Patient race White
Black/African American
Other/Unknown
Hispanic/Latino