Differences between human plasma and serum metabolite profiles.

Author(s): Yu Z, Kastenmüller G, He Y, Belcredi P, Möller G, Prehn C, Mendes J, Wahl S, Roemisch-Margl W, Ceglarek U, Polonikov A, Dahmen N, Prokisch H, Xie L, Li Y, Wichmann HE, Peters A, Kronenberg F, Suhre K, Adamski J, Illig T, Wang-Sattler R

Publication: PLoS One, 2011, Vol. 6, Page e21230

PubMed ID: 21760889 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of patient gender, smoking status, and a diagnosis of type 2 diabetes as well as blood component analyzed (EDTA plasma versus serum) on the concentrations of 122 metabolites. Specimens were drawn into either serum or EDTA plasma gel tubes, and plasma and serum were processed and frozen at -80 degrees C within 30-60 min of collection. Metabolic analysis was performed using the AbsoluteIDQ kit p150 and flow injection analysis mass spectrometry (FIA-MS).

   Summary of Findings:

   The concentrations of 122 metabolites showed strong correlations with previous results when analyses were repeated for both plasma (r=0.83) and serum specimens (r=0.80), but the reproducibility was significantly higher in plasma specimens (p=0.01). The concentrations of 85% of the 122 metabolites were significantly higher in serum than in plasma, but an overall strong correlation between the two types of specimens was observed (r=0.81). A greater number of metabolites were found to be differentially expressed between patients with type 2 diabetes and those without, males versus females, and smokers versus non-smokers when serum was analyzed rather than plasma.

   Biospecimens

   • Bodily Fluid - Plasma
   • Bodily Fluid - Serum
   • Bodily Fluid - Blood

   Preservative Types

   • Frozen

   Diagnoses:

   • Diabetes Type 2
   • Not specified

   Platform:

   Analyte	Technology Platform
   Lipid	LC-MS or LC-MS/MS
   Small molecule	LC-MS or LC-MS/MS
   Carbohydrate	LC-MS or LC-MS/MS

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Preaquisition	Other drugs	Smoker Non-smoker
   Preaquisition	Patient gender	Female Male
   Preaquisition	Diagnosis/ patient condition	Type 2 diabetes No type 2 diabetes
   Biospecimen Aliquots and Components	Blood and blood products	Plasma Serum

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