NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

Serum microRNAs are promising novel biomarkers.

Author(s): Gilad S, Meiri E, Yogev Y, Benjamin S, Lebanony D, Yerushalmi N, Benjamin H, Kushnir M, Cholakh H, Melamed N, Bentwich Z, Hod M, Goren Y, Chajut A

Publication: PLoS One, 2008, Vol. 3, Page e3148

PubMed ID: 18773077 PubMed Review Paper? No

Purpose of Paper

The purpose of this paper was to compare the microRNA (miRNA, miR) profiles of serum and urine and to determine the effects of room temperature storage, freeze-thaw cycling and pregnancy on levels of miRNA in serum.

Conclusion of Paper

The authors report the miRNA levels differed between serum and urine specimens. Serum levels of miRNA were not affected by storing serum at room temperature for 4 h versus 1 h or subjecting serum to 2 versus 1 freeze-thaw cycle. Many miRNA including the placental miRNAs (miR-527, -526a and -520d-5p) were expressed at higher levels in the sera of pregnant women then non-pregnant women, and levels of the placental miRNA increased during pregnancy.

Studies

  1. Study Purpose

    The purpose of this study was to compare the miRNA profiles of serum and urine and to determine the effects of room temperature storage, freeze-thaw cycling and pregnancy on levels of miRNA in serum. Serum and urine were collected from 10 non-pregnant women and 20 pregnant women (10 6-12 weeks pregnant and 10 34-41 weeks pregnant) and stored at -80°C. RNA was extracted with phenol-chloroform and stored at -20°C. The authors mention expression of miRNA in saliva, amniotic fluid and pleural fluid, but the details of specimen collection and the results were not specified.

    Summary of Findings:

    The authors report that miRNA was detectable in urine, saliva, amniotic fluid and pleural fluid and that levels of miRNA differed between serum and urine specimens. Serum levels of miRNA were not affected by 1 versus 4 h of storage at room temperature or 1 versus 2 freeze-thaw cycles. Pregnant women in the third trimester had significantly higher levels of miR-526a (694-fold, p=2.1x10^-7), miR-527 (622-fold, p=1.2 x 10^-14), miR-515-5p (511-fold, p=6.9 x 10^-8), miR-521 (164-fold, p=8.1 x 10^-9), miR-523 (28-fold, p=2.2 x 10^-6), miR-524* (27-fold, p=0.0028), miR518a-3p (12-fold, p=0.00018), miR-520d-5p (8.6-fold, p=3.3 x 10^-7), miR525-3p (6.6-fold, p=0.00056), miR-519e (5.2-fold, p=00.00013), miR-518d (5.1-fold, p=0.0076), miR-524 (4.8-fold, p=0.0038), miR-512-3p (4.5-fold, p=0.0019), miR-141 (4.0-fold, p=0.00039), miR-519d (3.7-fold, p=0.026), miR-517 (3.5-fold, p=0.058), miR-518e (2.8 fold, p=0.035), and miR-145 (2-fold, p=0.032) than non-pregnant women. Levels of miR-527, miR-520d-5p, and miR-526a  were higher in specimens obtained during the third trimester than the first trimester, but the significance was not determined. Levels of miR-149, let-7d, miR-16, miR-126, miR-572, miR-202 and miR-451 were not affected by pregnancy.

    Biospecimens
    Preservative Types
    • Frozen
    Diagnoses:
    • Normal
    • Pregnant
    Platform:
    AnalyteTechnology Platform
    RNA Real-time qRT-PCR
    Pre-analytical Factors:
    ClassificationPre-analytical FactorValue(s)
    Preaquisition Diagnosis/ patient condition Not pregnant
    6-12 weeks pregnant
    34-41 weeks pregnant
    Storage Freeze/thaw cycling 1 cycle
    2 cycles
    Storage Time at room temperature 1 h
    4 h
    Biospecimen Acquisition Biospecimen location Urine
    Serum

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