Cancer Diagnosis Program | Biorepositories and Biospecimen Research Branch

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Serum microRNAs are promising novel biomarkers.

Author(s): Gilad S, Meiri E, Yogev Y, Benjamin S, Lebanony D, Yerushalmi N, Benjamin H, Kushnir M, Cholakh H, Melamed N, Bentwich Z, Hod M, Goren Y, Chajut A

Publication: PLoS One, 2008, Vol. 3, Page e3148

PubMed ID: 18773077 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to compare the miRNA profiles of serum and urine and to determine the effects of room temperature storage, freeze-thaw cycling and pregnancy on levels of miRNA in serum. Serum and urine were collected from 10 non-pregnant women and 20 pregnant women (10 6-12 weeks pregnant and 10 34-41 weeks pregnant) and stored at -80°C. RNA was extracted with phenol-chloroform and stored at -20°C. The authors mention expression of miRNA in saliva, amniotic fluid and pleural fluid, but the details of specimen collection and the results were not specified.

   Summary of Findings:

   The authors report that miRNA was detectable in urine, saliva, amniotic fluid and pleural fluid and that levels of miRNA differed between serum and urine specimens. Serum levels of miRNA were not affected by 1 versus 4 h of storage at room temperature or 1 versus 2 freeze-thaw cycles. Pregnant women in the third trimester had significantly higher levels of miR-526a (694-fold, p=2.1x10^-7), miR-527 (622-fold, p=1.2 x 10^-14), miR-515-5p (511-fold, p=6.9 x 10^-8), miR-521 (164-fold, p=8.1 x 10^-9), miR-523 (28-fold, p=2.2 x 10^-6), miR-524* (27-fold, p=0.0028), miR518a-3p (12-fold, p=0.00018), miR-520d-5p (8.6-fold, p=3.3 x 10^-7), miR525-3p (6.6-fold, p=0.00056), miR-519e (5.2-fold, p=00.00013), miR-518d (5.1-fold, p=0.0076), miR-524 (4.8-fold, p=0.0038), miR-512-3p (4.5-fold, p=0.0019), miR-141 (4.0-fold, p=0.00039), miR-519d (3.7-fold, p=0.026), miR-517 (3.5-fold, p=0.058), miR-518e (2.8 fold, p=0.035), and miR-145 (2-fold, p=0.032) than non-pregnant women. Levels of miR-527, miR-520d-5p, and miR-526a  were higher in specimens obtained during the third trimester than the first trimester, but the significance was not determined. Levels of miR-149, let-7d, miR-16, miR-126, miR-572, miR-202 and miR-451 were not affected by pregnancy.

   Biospecimens

   • Bodily Fluid - Serum
   • Bodily Fluid - Blood
   • Bodily Fluid - Urine
   • Bodily Fluid - Amniotic Fluid
   • Bodily Fluid - Pleural Fluid
   • Bodily Fluid - Saliva

   Preservative Types

   • Frozen

   Diagnoses:

   • Normal
   • Pregnant

   Platform:

   Analyte	Technology Platform
   RNA	Real-time qRT-PCR

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Preaquisition	Diagnosis/ patient condition	Not pregnant 6-12 weeks pregnant 34-41 weeks pregnant
   Storage	Freeze/thaw cycling	1 cycle 2 cycles
   Storage	Time at room temperature	1 h 4 h
   Biospecimen Acquisition	Biospecimen location	Urine Serum

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