Serum microRNAs are promising novel biomarkers.
Author(s): Gilad S, Meiri E, Yogev Y, Benjamin S, Lebanony D, Yerushalmi N, Benjamin H, Kushnir M, Cholakh H, Melamed N, Bentwich Z, Hod M, Goren Y, Chajut A
Publication: PLoS One, 2008, Vol. 3, Page e3148
PubMed ID: 18773077 PubMed Review Paper? No
Purpose of Paper
The purpose of this paper was to compare the microRNA (miRNA, miR) profiles of serum and urine and to determine the effects of room temperature storage, freeze-thaw cycling and pregnancy on levels of miRNA in serum.
Conclusion of Paper
The authors report the miRNA levels differed between serum and urine specimens. Serum levels of miRNA were not affected by storing serum at room temperature for 4 h versus 1 h or subjecting serum to 2 versus 1 freeze-thaw cycle. Many miRNA including the placental miRNAs (miR-527, -526a and -520d-5p) were expressed at higher levels in the sera of pregnant women then non-pregnant women, and levels of the placental miRNA increased during pregnancy.
The purpose of this study was to compare the miRNA profiles of serum and urine and to determine the effects of room temperature storage, freeze-thaw cycling and pregnancy on levels of miRNA in serum. Serum and urine were collected from 10 non-pregnant women and 20 pregnant women (10 6-12 weeks pregnant and 10 34-41 weeks pregnant) and stored at -80°C. RNA was extracted with phenol-chloroform and stored at -20°C. The authors mention expression of miRNA in saliva, amniotic fluid and pleural fluid, but the details of specimen collection and the results were not specified.
Summary of Findings:
The authors report that miRNA was detectable in urine, saliva, amniotic fluid and pleural fluid and that levels of miRNA differed between serum and urine specimens. Serum levels of miRNA were not affected by 1 versus 4 h of storage at room temperature or 1 versus 2 freeze-thaw cycles. Pregnant women in the third trimester had significantly higher levels of miR-526a (694-fold, p=2.1x10^-7), miR-527 (622-fold, p=1.2 x 10^-14), miR-515-5p (511-fold, p=6.9 x 10^-8), miR-521 (164-fold, p=8.1 x 10^-9), miR-523 (28-fold, p=2.2 x 10^-6), miR-524* (27-fold, p=0.0028), miR518a-3p (12-fold, p=0.00018), miR-520d-5p (8.6-fold, p=3.3 x 10^-7), miR525-3p (6.6-fold, p=0.00056), miR-519e (5.2-fold, p=00.00013), miR-518d (5.1-fold, p=0.0076), miR-524 (4.8-fold, p=0.0038), miR-512-3p (4.5-fold, p=0.0019), miR-141 (4.0-fold, p=0.00039), miR-519d (3.7-fold, p=0.026), miR-517 (3.5-fold, p=0.058), miR-518e (2.8 fold, p=0.035), and miR-145 (2-fold, p=0.032) than non-pregnant women. Levels of miR-527, miR-520d-5p, and miR-526a were higher in specimens obtained during the third trimester than the first trimester, but the significance was not determined. Levels of miR-149, let-7d, miR-16, miR-126, miR-572, miR-202 and miR-451 were not affected by pregnancy.
- Bodily Fluid - Serum
- Bodily Fluid - Blood
- Bodily Fluid - Urine
- Bodily Fluid - Amniotic Fluid
- Bodily Fluid - Pleural Fluid
- Bodily Fluid - Saliva
Analyte Technology Platform RNA Real-time qRT-PCR
Classification Pre-analytical Factor Value(s) Preaquisition Diagnosis/ patient condition Not pregnant
6-12 weeks pregnant
34-41 weeks pregnant
Storage Freeze/thaw cycling 1 cycle
Storage Time at room temperature 1 h
Biospecimen Acquisition Biospecimen location Urine