Which is the best anticoagulant for whole blood aggregometry platelet function testing? Comparison of six anticoagulants and diverse storage conditions.

Author(s): Kaiser AF, Neubauer H, Franken CC, Krüger JC, Mügge A, Meves SH

Publication: Platelets, 2012, Vol. 23, Page 359-67

PubMed ID: 21999185 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of anticoagulant type, storage duration and temperature, and stimulating agonist on platelet aggregation.

   Summary of Findings:

   Baseline values of ADP- or AA-induced aggregometry were not significantly different between blood collected in sodium heparin, lithium heparin, r-hirudin (20 or 45 ug/mL), or BAPA. However, specimens collected in citrate had significantly lower baseline values for both stimulating agonists. With storage of blood at room temperature prior to assay, all specimens, except those collected in BAPA, showed increases in ADP-induced aggregometry values in the first 30-120 min and declined thereafter. ADP-induced values for BAPA specimens declined throughout room temperature storage. Nevertheless, ADP-induced results remained stable for up to 8 h at room temperature in specimens collected in lithium heparin or 45 ug/mL r-hirudin, and for up to 12 h in specimens collected in sodium heparin. AA-induced platelet aggregation values were somewhat more stable during room temperature storage of blood than ADP-induced values, remaining stable for up to 12 h in specimens collected in 45 ug/mL r-hirudin and for 24 h in specimens collected in sodium heparin. When BAPA and r-hirudin (20 ug/mL) specimens were stored at either 4 degrees C or 37 degrees C rather than room temperature, the stability of both ADP and AA-induced aggregometry results was generally worse than when specimens were stored at room temperature.

   Biospecimens

   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Cell count/volume	Hematology/ auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Anticoagulant	Lithium heparin Sodium heparin Hirudin Multiple concentrations evaluated BAPA Citrate
   Hematology/ auto analyzer Specific	Reaction solution	ADP AA
   Storage	Storage temperature	Room temperature 4 degrees C 37 degrees C
   Storage	Storage duration	0 min 30 min 1 h 2 h 4 h 6 h 8 h 12 h 24 h 36 h 48 h

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