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Pre-analytical effects of pneumatic tube transport on impedance platelet aggregometry.

Author(s): Bolliger D, Seeberger MD, Tanaka KA, Dell-Kuster S, Gregor M, Zenklusen U, Grapow M, Tsakiris DA, Filipovic M

Publication: Platelets, 2009, Vol. 20, Page 458-65

PubMed ID: 19852683 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of PTS transport on platelet function in specimens from patients scheduled for coronary artery bypass. 3 specimens were taken simultaneously from the arterial line of patients, prior to anesthesia, but while on aspirin therapy. The reference specimens were collected first followed by the control specimens both of which were analyzed in the operating room 35-45 min after sampling, but half of the control specimens were transported through the PTS prior to analysis while the reference specimens and other half of the control specimens remained at room temperature. The third specimen (laboratory) was analyzed by another individual approximately 30 min after transport (3-5 min) to the central laboratory via PTS.

   Summary of Findings:

   TRAP-induced impedance aggregometry was significantly lower in the control (transported or not) and laboratory specimens than the reference specimens (p<0.05), but ASPI-induced impedance aggregometry was only significantly reduced after PTS transport (control or laboratory). Further, while 96% of the reference specimens and 88% of the control specimens that did not undergo PTS transport had normal platelet function, only 60% of control specimens transported by the PTS and 70% of laboratory specimens had normal platelet function (both p<0.05). While 14% of reference specimens showed reduced aspirin responsiveness, 4% of control specimens that were not subjected to PTS transport, 0% of control specimens that were subjected to PTS trasnport and 2% of laboratory specimens showed reduced aspirin responsiveness (all p<0.05). The authors conclude that PTS transport significantly affects platelet function as assessed by both TRAP and ASPI-induced impedance aggregometry.

   Biospecimens

   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Cardiovascular Disease

   Platform:

   Analyte	Technology Platform
   Morphology	Hematology/ auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Hematology/ auto analyzer Specific	Reaction solution	Thrombin-related activation peptide Arachidonic acid
   Storage	Within hospital transportation method	Not transported Pneumatic tube system

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