Diurnal variation in platelet aggregation iwth the PFA-100 platelet function analyser.

Author(s): Dalby MC, Davidson SJ, Burman JF, Davies SW

Publication: Platelets, 2000, Vol. 11, Page 320-4

PubMed ID: 11083456 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of time of biospecimen collection on platelet aggregation measured by a PFA-100 platelet function analyzer. Chemical agonists collagen and either ADP or ephinephrine were used, and the time before the experimental aperture was occluded was measured.

   Summary of Findings:

   With epinephrine, a non-significant decrease in closure time was observed in specimens collected at 8:30 am, after volunteers had risen, compared to closure times observed in specimens collected from supine patients at 8:00 am, prior to rising. With ADP, there was also a non-significant decrease in closure time in specimens collected at 8:30 am, after volunteers had risen, compared to that measured in specimens from supine patients collected at 8:00 am, prior to rising. Specimens collected at 8:30 am had shorter epinephrine and ADP stimulated closure times than those collected at 5:00 pm (p=0.0023, p=0.03, respectively), but this difference was less marked with ADP than with epinephrine.

   Biospecimens

   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Cell count/volume	Hematology/ auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Time of biospecimen collection	8:00 am (supine) 8:30 am (after rising) 12:00 pm 5:00 pm
   Hematology/ auto analyzer Specific	Reaction solution	Collagen and ADP Collagen and epinephrine

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