Do evacuated blood collection tubes interfere with therapeutic drug monitoring?

Author(s): Janknegt R, Lohman JJ, Hooymans PM, Merkus FW

Publication: Pharm Weekbl Sci, 1983, Vol. 5, Page 287-90

PubMed ID: 6664820 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of collection tube type on serum levels of therapeutic drugs. Patient blood was collected in syringes, transferred to the different tubes, and inverted ten times prior to serum separation and freezing.

   Summary of Findings:

   Collection tube type did not effect ethosuximide, phenobarbital, phenytoin, valproic acid, digitoxin, digoxin, procainamide, N-acetylprocainamide, gentamicin or theophylline levels in serum. Further, all drug levels remained within the normal therapeutic range, regardless of tube type. When serum was collected in red stoppered Monoject tubes, significantly lower carbamazepine (p<0.01) and disopyramide (p<0.05) levels were found compared to specimens collected in glass tubes. Guinidine levels were significantly lower in serum collected in red stoppered Vacutainer (p<0.001) or Monoject tubes (p<0.02) than in serum collected in glass tubes. No significant differences were found when serum was collected in blue stoppered Vacutainer tubes or red stoppered Venoject tubes in comparison to serum collected in glass tubes.

   Biospecimens

   • Bodily Fluid - Serum
   • Bodily Fluid - Blood

   Preservative Types

   • Frozen

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Small molecule	HPLC
   Small molecule	Radioimmunoassay
   Small molecule	Fluorometry
   Small molecule	Immunoassay
   Small molecule	GC- flame ionization

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Type of collection container/solution	Red stoppered Vacutainer Blue stoppered Vacutainer Red stoppered Monoject Red stoppered Venoject Open glass tube

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