Cancer Diagnosis Program | Biorepositories and Biospecimen Research Branch

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Comparison of single and double centrifugation methods for preparation of Platelet-Rich Plasma (PRP).

Author(s): Saqlain N, Mazher N, Fateen T, Siddique A

Publication: Pak J Med Sci, 2023, Vol. 39, Page 634-637

PubMed ID: 37250535 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   This study compared platelet, RBC, and WBC counts in plasma obtained by a single centrifugation step at 3500 x g and plasma obtained by centrifugation at 1000 x g followed by 800 x g. Blood was collected from fifty volunteers (age: 20-45 years) into tri-sodium citrate syringes and transferred to tubes. Plasma was separated from matched tubes by single centrifugation at 3500 x g for 10 min, and by double centrifugation at 1000 x g for 5 min followed by centrifugation at 800 x g for 10 min. Platelet and cell counts were enumerated in whole blood and plasma using a SYSMEX hematology analyzer.

   Summary of Findings:

   Platelet counts and the percentage of platelets retained in the plasma (platelet yield) were higher in plasma obtained by double centrifugation (1000 x g for 5 min followed by 800 x g for 10 min) than by single centrifugation (3500 x g for 10 min) (P<0.01, both). However, WBC counts were higher in single-spin plasma than double-spin plasma (6.05 x 10³ versus 1.06 x 10³ per µL, P<0.01), but there was no difference in RBC counts.

   Biospecimens

   • Bodily Fluid - Plasma
   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified
   • Normal

   Platform:

   Analyte	Technology Platform
   Cell count/volume	Hematology/ auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Aliquots and Components	Centrifugation	Different number of centrifugation steps compared Multiple speeds compared

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