Influence of age, postmortem delay and freezing storage period on cannabinoid receptor density and functionality in human brain.

Author(s): Mato S, Pazos A

Publication: Neuropharmacology, 2004, Vol. 46, Page 716

PubMed ID: 14996549 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   To assess the effect of patient age (23-77 yr) on detectable CB receptor density and G-protein activation.

   Summary of Findings:

   Both CB receptor density and G-protein activation (via an agonist) were negatively correlated with patient age. Both variables decreased by approximately 10% per decade.

   Biospecimens

   • Tissue - Brain

   Preservative Types

   • Frozen

   Diagnoses:

   • Neoplastic
   • Normal
   • Autopsy

   Platform:

   Analyte	Technology Platform
   Protein	Radioimmunoassay

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Preaquisition	Patient age	22 years 73 years

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2. Study Purpose

   To assess the effect of a post mortem delay at 4 degrees C for up to 66 h on detectable CB receptor density and G-protein activation.

   Summary of Findings:

   CB receptor density and G-protein activation were not affected by a postmortem delay at 4 degrees C for 66 h.

   Biospecimens

   • Tissue - Brain

   Preservative Types

   • Frozen

   Diagnoses:

   • Autopsy
   • Neoplastic
   • Normal

   Platform:

   Analyte	Technology Platform
   Protein	Radioimmunoassay

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Preaquisition	Postmortem interval	0 h 66 h

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3. Study Purpose

   To assess the effects of frozen storage duration (-25 degrees C) on detectable CB receptor density and G-protein activation.

   Summary of Findings:

   CB receptor density exponentially decreased with the duration of frozen storage (-25 degrees C), with a 50% reduction observed between 12-24 months of storage. G-protein basal and activated levels were negatively correlated with the duration of frozen storage, indicating that G-protein coupling ability was impaired.

   Biospecimens

   • Tissue - Brain

   Preservative Types

   • Frozen

   Diagnoses:

   • Autopsy
   • Neoplastic
   • Normal

   Platform:

   Analyte	Technology Platform
   Protein	Radioimmunoassay

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage duration	0 months 2 months 12 months 24 months 91 months

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