Cancer Diagnosis Program | Biorepositories and Biospecimen Research Branch

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MicroRNAs accurately identify cancer tissue origin.

Author(s): Rosenfeld N, Aharonov R, Meiri E, Rosenwald S, Spector Y, Zepeniuk M, Benjamin H, Shabes N, Tabak S, Levy A, Lebanony D, Goren Y, Silberschein E, Targan N, Ben-Ari A, Gilad S, Sion-Vardy N, Tobar A, Feinmesser M, Kharenko O, Nativ O, Nass D, Perelman M, Yosepovich A, Shalmon B, Polak-Charcon S, Fridman E, Avniel A, Bentwich I, Bentwich Z, Cohen D, Chajut A, Barshack I

Publication: Nat Biotechnol, 2008, Vol. 26, Page 462-9

PubMed ID: 18362881 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of storage of lung tumor FFPE blocks and tumor tissue origin on miRNA expression in 333 FFPE tumor blocks from 22 different tissue types. RNA was obtained from 7-10 10 uM sections by xylene deparaffinization, extraction with the miRdictor kit, and miRNA profile was determined using custom miRdicator arrays. The ability of the 48 identified miRNA to function as tumor classifiers was tested by blinded analysis of 83 FFPE tumor blocks.

   Summary of Findings:

   Overall miRNA expression of any one lung cancer specimen stored for 2-11 years showed a correlation of 0.9 with the median expression of the 13 specimens. The miRNA profile of most cancers was similar between primary and metastasis, but this was not true for stomach cancers where 3 miRNAs were differentially expressed. 48 miRNAs were identified as tumor classifiers and validated using real-time PCR.

   Biospecimens

   • Tissue - Lung
   • Tissue - Stomach
   • Tissue - Breast
   • Tissue - Colorectal
   • Tissue - Endometrium
   • Tissue - Head and Neck
   • Tissue - Kidney
   • Tissue - Brain
   • Tissue - Bladder
   • Tissue - Liver
   • Tissue - Lymph Node
   • Tissue - Skin
   • Tissue - Ovary
   • Tissue - Pancreas
   • Tissue - Prostate
   • Tissue - Small Bowel
   • Tissue - Testis
   • Tissue - Thyroid Gland
   • Tissue - Thymus Gland

   Preservative Types

   • Formalin

   Diagnoses:

   • Neoplastic - Carcinoma

   Platform:

   Analyte	Technology Platform
   RNA	Real-time qRT-PCR
   RNA	DNA microarray

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage duration	2-11 years
   Preaquisition	Diagnosis/ patient condition	Primary tumor Metastatic tumor

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