Cancer Diagnosis Program | Biorepositories and Biospecimen Research Branch

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p53 antigen loss in stored paraffin slides.

Author(s): Prioleau J, Schnitt SJ

Publication: N Engl J Med, 1995, Vol. 332, Page 1521-2

PubMed ID: 7739705 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this paper was to determine if p53 immunoreactivity diminishes with room temperature storage of slide-mounted formalin-fixed paraffin-embedded (FFPE) tissue sections. Slide-mounted FFPE sections containing mammary ductal carcinoma specimens from ten patients were analyzed immediately or stored at room temperature for 2 months.

   Summary of Findings:

   In all ten cases examined, p53 immunostaining was diminished or absent in slides stored at room temperature for 2 months compared to freshly sectioned case-matched controls.

   Biospecimens

   • Tissue - Breast

   Preservative Types

   • Formalin

   Diagnoses:

   • Neoplastic - Carcinoma

   Platform:

   Analyte	Technology Platform
   Protein	Immunohistochemistry

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage duration	Fresh (duration not specified) 2 months
   Immunohistochemistry Specific	Targeted peptide/protein	p53

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