Lysis of whole blood in vitro causes DNA strand breaks in human lymphocytes.
Author(s): Narayanan S, O'Donovan M RO, Duthie S J
Publication: Mutagenesis, 2001, Vol. 16, Page 455
PubMed ID: 11682634 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
-
Study Purpose
To determine if DNA damage in lymphocytes is influenced by culture in whole blood.
Summary of Findings:
DNA damage was 10-fold greater in whole blood specimens compared to lymphocytes isolated from whole blood post collection.
Biospecimens
Preservative Types
- None (Fresh)
Diagnoses:
- Not specified
Platform:
Analyte Technology Platform DNA Comet assay Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Aliquots and Components Blood and blood products Lymphocyte
Whole blood
-
Study Purpose
To determine if DNA damage in lymphocytes is influenced by the storage conditions (duration and temperature) of whole blood specimens.
Summary of Findings:
Storage of whole blood for 24 h (at 4 degrees C or room temperature) significantly induced DNA damage, which was further elevated after 48 h in specimens stored at room temperature.
Biospecimens
Preservative Types
- None (Fresh)
Diagnoses:
- Not specified
Platform:
Analyte Technology Platform DNA Comet assay Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Storage temperature Room temperature
4 degrees C
Storage Storage duration 0 h
24 h
48 h
-
Study Purpose
To assess comparative differences in DNA damage among specimens with different levels of red blood cell contamination risk: 1) lymphocytes grown in mononuclear culture, 2) lymphocytes isolated from whole blood post collection, and 3) lymphocytes cultured in whole blood.
Summary of Findings:
While lymphocytes grown in mononuclear cell culture and those isolated from whole blood had comparable levels of DNA damage on days 0 and 1, the latter displayed significantly higher levels of DNA damage on day 2. DNA damage in lymphocytes isolated from whole blood and those cultured in whole blood increased as a function of time. Lymphocytes cultured in granulocyte depleted blood displayed intermediate levels of DNA damage in comparison to those grown in mononuclear culture or whole blood, indicating that a whole blood constituent in addition to granulocytes influences lymphocyte DNA damage.
Biospecimens
Preservative Types
- None (Fresh)
Diagnoses:
- Not specified
Platform:
Analyte Technology Platform Cell count/volume Flow cytometry DNA Comet assay Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Storage duration 0 days
1 day
2 days
3 days
4 days
7 days
Biospecimen Aliquots and Components Blood and blood products Lymphocytes
Whole blood