Cancer Diagnosis Program | Biorepositories and Biospecimen Research Branch

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Dual-color silver-enhanced in situ hybridization for assessing HER2 gene amplification in breast cancer.

Author(s): Koh YW, Lee HJ, Lee JW, Kang J, Gong G

Publication: Mod Pathol, 2011, Vol. 24, Page 794-800

PubMed ID: 21317877 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to compare the determination of HER2 status by single- and dual-color SISH and FISH in invasive breast carcinoma specimens. Discrepant cases were also assessed by immunohistochemistry (IHC).

   Summary of Findings:

   High HER2 status concordance rates were demonstrated between dual-color SISH and FISH (141/146 cases, 97%) and dual-color SISH and single-color SISH (142/146 cases, 97%). Of the 7 cases showing any discordance between analytical methods, HER2 status determined by IHC was in concordance with the FISH results in 5 cases.

   Biospecimens

   • Tissue - Breast

   Preservative Types

   • Formalin

   Diagnoses:

   • Neoplastic - Carcinoma

   Platform:

   Analyte	Technology Platform
   DNA	FISH
   DNA	Tissue microarray
   Protein	Immunohistochemistry
   Protein	Tissue microarray
   DNA	In situ hybridization

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   In situ hybridization Specific	Technology platform	FISH Single-color SISH Dual-color SISH Immunohistochemistry

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