Comparison of fluorescent in situ hybridization HER-2/neu results on core needle biopsy and excisional biopsy in primary breast cancer.
Author(s): Apple SK, Lowe AC, Rao PN, Shintaku IP, Moatamed NA
Publication: Mod Pathol, 2009, Vol. 22, Page 1151-9
PubMed ID: 19483670 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to compare FISH and IHC analysis of HER-2/neu from core needle and excisional biopsies of breast carcinoma specimens. Specimens were collected from 125 patients, 5 of which had received neoadjuvant chemotheraphy.
Summary of Findings:
In 87% of specimens, there was complete concordance between FISH and IHC results and excisional and core needle biopsy specimens. Concordance was found between FISH and IHC results within a specimen 95% of the time, with most of the discordant specimens showing no IHC staining despite amplification by FISH. Core needle and excisional biopsies were concordant 92% of the time with FISH analysis and 98% of the time with IHC analysis. In 5 cases in which neoadjuvant chemotherapy was received, no differences between FISH or IHC were observed nor were there differences between core needle and excisional biopsy. The authors conclude that IHC is slightly more consistent than FISH, and that it is reasonable to examine both the core and excisional biopsy specimens especially in cases where the tumor characteristics are found to be different in the core needle and excisional biopsy.
Biospecimens
Preservative Types
- Other Preservative
Diagnoses:
- Neoplastic - Carcinoma
Platform:
Analyte Technology Platform Protein Immunohistochemistry Morphology H-and-E microscopy DNA FISH Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Immunohistochemistry Specific Targeted peptide/protein HER-2/neu
FISH Specific Targeted nucleic acid HER-2/neu
FISH Specific Technology platform FISH
Immunohistochemistry
Biospecimen Acquisition Method of tissue acquisition Core needle biopsy
Excisional biopsy