NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis
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Do prognostic marker studies on core needle biopsy specimens of breast carcinoma accurately reflect the marker status of the tumor?

Author(s): Jacobs TW, Siziopikou KP, Prioleau JE, Raza S, Baum JK, Hayes DF, Schnitt SJ

Publication: Mod Pathol, 1998, Vol. 11, Page 259-64

PubMed ID: 9521472 PubMed Review Paper? No

Purpose of Paper

The purpose of this paper was to compare immunohistochemical (IHC) staining of core needle biopsy (CNB) and surgical excision (SE) breast cancer specimens.

Conclusion of Paper

B-cell lymphoma 2 (Bcl-2), estrogen receptor (ER), human epidermal growth factor receptor 2 (HER2), and p53 immunostaining results were completely concordant between all paired CNB and SE specimens. A significant correlation was observed between the microvessel density (MVD) in CNB and SE specimens, but in 42 of 49 cases, the average MVD on the CNB differed by more than 10% from the average MVD on the SE specimen. The number of 224x fields evaluated to determine MVD did not affect the percentage difference between CNB and SE specimens. Further, the authors state that no correlations were found between needle gauge or number of passes during the CNB and the percentage difference in MVD between CNB and SE specimens.

Studies

  1. Study Purpose

    The purpose of this study was to compare IHC staining in CNB and SE breast cancer specimens. The time between acquisition of CNB and SE specimens ranged from 4-47 days. Slides were scored either as positive or negative for Bcl-2, ER, HER2, and p53, while factor VIII-related antigen immunostaining was used to determine MVD.

    Summary of Findings:

    Bcl-2, ER, HER2, and p53 immunostaining results were completely concordant between all paired CNB and SE specimens (54-55 pairs). A significant correlation was observed between the MVD in CNB and SE specimens (p=0.0002), but in 42 of 49 cases, the average MVD on the CNB differed by more than 10% from the average MVD on the SE specimen. In 30 of 49 cases, MVD was higher in the CNB than the SE specimen, and in the remaining 19 cases, MVD was higher in the SE specimen. The number of 224x fields evaluated to determine MVD did not affect the percentage difference between CNB and SE specimens. Further, the authors state that no correlations were found between needle gauge or number of passes during CNB and the percentage difference in MVD between CNB and SE specimens.

    Biospecimens
    Preservative Types
    • Formalin
    Diagnoses:
    • Neoplastic - Carcinoma
    Platform:
    AnalyteTechnology Platform
    Protein Immunohistochemistry
    Morphology Light microscopy
    Pre-analytical Factors:
    ClassificationPre-analytical FactorValue(s)
    Immunohistochemistry Specific Targeted peptide/protein Bcl-2
    ER
    HER2
    p53
    Factor VIII-related antigen
    Biospecimen Aliquots and Components Aliquot size/volume At least 3 224x microscope fields were evaluated
    1-2 224x microscope fields were evaluated
    Biospecimen Acquisition Needle gauge 13-18-gauge needle
    14-gauge needle
    Biospecimen Aliquots and Components Number of cell passages 3-6 passes
    5-6 passes
    Biospecimen Acquisition Method of tissue acquisition Core needle biopsy
    Surgical resection
    View more

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