Influence of tube type on the antiglobulin test.

Author(s): Black D, Kay J

Publication: Med Lab Sci, 1986, Vol. 43, Page 169-73

PubMed ID: 3736368 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of tube type, reagent source, centrifugation speed (400 x g versus 1000 x g) and antibody on AHG in red blood cells.

   Summary of Findings:

   Generally the strongest AHG reactions were observed with the IMMUNO reagents and the weakest with the Blood Group Reference Laboratory (BGRL) reagents. Regardless of reagent source and antibody, AHG reactions increased with faster centrifugation and when the reaction occurred in glass rather than in plastic tubes. The decreased reaction in plastic tubes was found to be due to diminished anti-immunoglobulin G (anti-IgG) activity and not a change in anti-C3 activity. The effect was reduced as the concentration of antibody increased, but only occurred when both washing and the reaction were done in the plastic tube.

   Biospecimens

   • Cell - Red Blood Cells

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Protein	Antiglobulin test
   Protein	Macroscopic observation

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Antiglobulin test Specific	Antibody dilution/concentration	1:50 anti-IgG 1:75 anti-IgG 1:80 anti-IgG 1:100 anti-IgG
   Antiglobulin test Specific	Targeted peptide/protein	Anti-C3 Anti-IgG Anti-Fy Anti-Jk Anti-D Anti-s Anti-Le Anti-c Anti-S Anti-K
   Antiglobulin test Specific	Technology platform	Scottish Antibody Production Unit IMMUNO Blood Group Reference Laboratory
   Storage	Type of storage container	Glass Plastic
   Biospecimen Aliquots and Components	Centrifugation	Multiple speeds compared

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