Effect of long-term freezer storage, thawing, and refreezing on selected constituents of serum.
Author(s): DiMagno EP, Corle D, O'Brien JF, Masnyk IJ, Go VL, Aamodt R
Publication: Mayo Clin Proc, 1989, Vol. 64, Page 1226-34
PubMed ID: 2593713 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to evaluate the effects of frozen storage for up to 10 years and up to 6 freeze-thaw cycles on electrolytes, hormones, vitamins, immunoglobulins, enzymes, and tumor markers in serum.
Summary of Findings:
After storage for up to 10 years, only very small changes in analyte values which were likely to be clinically insignificant were observed. While electrolytes tended to increase with storage, enzymes tended to decrease, but the values remained within normal ranges. Similarly, freeze-thaw cycling generally resulted in small clinically insignificant changes. Lactate dehydrogenase (LDH) and creatinine kinase (CK) concentrations were both decreased when specimens underwent 4 freeze-thaw cycles where thawing occurred at room temperature for 4 h. LDH was also decreased in specimens after 4 freeze-thaw cycles with a 30 minute thaw at room temperature.
Biospecimens
Preservative Types
- Frozen
Diagnoses:
- Neoplastic - Benign
- Normal
Platform:
Analyte Technology Platform Electrolyte/Metal Clinical chemistry/auto analyzer Protein Clinical chemistry/auto analyzer Small molecule Clinical chemistry/auto analyzer Peptide Clinical chemistry/auto analyzer Steroid Clinical chemistry/auto analyzer Glycoprotein Clinical chemistry/auto analyzer Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Storage temperature -70 degrees C
Storage Storage duration 1 year
2 years
3 years
4 years
5 years
6 years
7 years
8 years
9 years
10 years
Storage Freeze/thaw cycling 0 cycles
1 cycle
2 cycles
4 cycles
6 cycles
Storage Thaw duration 30 min
4 h