Venous occlusion and estimation of serum constituents.

Author(s): Crombie IK, Smith WC, Tavendale RT, Clark EC, Tunstall-Pedoe HD

Publication: Lancet, 1987, Vol. 2, Page 975

PubMed ID: 2889899 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of tourniquet time and tourniquet pressure on total cholesterol, HDL cholesterol, GGT, triglycerides, creatinine, thiocyanate, uric acid, and glucose in serum. Either a blood pressure cuff or a Velcro tourniquet was used, and specimens were withdrawn sequentially at time 0 (before venous occlusion), and after 1, 2, and 3 min of venous occlusion. The first 1.5 mL was discarded at each time point. The authors do not give details about serum preparation or storage prior to analysis.

   Summary of Findings:

   No significant differences in any of the 8 serum constituents were noted when a blood pressure cuff was applied at 20 mm Hg for 1, 2, or 3 minutes prior to blood draw or when a Velcro tourniquet was used compared to the levels measured in specimens drawn with no venous occlusion. However, when the blood pressure cuff was applied at 40 mm Hg for 3 min during specimen collection, significant increases in HDL cholesterol (+7.11%), total cholesterol (+5.71%), GGT (+4.15%), and triglycerides (+3.96%), as well as significant decreases in glucose levels (-4.70%) were measured compared to levels measured in specimens drawn prior to venous occlusion. These trends were linear during 1-3 min of venous occlusion. Creatinine, thiocyanate, and uric acid were not significantly affected by venous occlusion at any pressure or for any duration tested.

   Biospecimens

   • Bodily Fluid - Serum
   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Steroid	Clinical chemistry/auto analyzer
   Protein	Clinical chemistry/auto analyzer
   Lipid	Clinical chemistry/auto analyzer
   Small molecule	Clinical chemistry/auto analyzer
   Carbohydrate	Clinical chemistry/auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Method of fluid acquisition	Tourniquet pressures compared Tourniquet times compared

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