Room temperature activates human blood platelets.

Author(s): Maurer-Spurej E, Pfeiler G, Maurer N, Lindner H, Glatter O, Devine DV

Publication: Lab Invest, 2001, Vol. 81, Page 581-92

PubMed ID: 11304578 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of storage temperature on platelet activation in PRP. 3-5 specimens were included in each experiment.

   Summary of Findings:

   Storage of PRP for 10 min at 20 degrees C, but not 42 degrees C, resulted in a morphological change in the platelets as determined by light microscopy and static and dynamic light scattering. The use of ACD, instead of sodium citrate, decreased platelet activation, and incubation for 15 min at 37 degrees C restored the morphology, regardless of storage time at 20 degrees C. The hydrodynamic radius was similar in platelets incubated at 30, 34 and 37 degrees C, but increased progressively as temperatures decreased below 30 degrees C. Flow cytometry confirmed a resting phenotype for platelets incubated at 37 or 42 degrees C, but after incubation at 20 degrees C, there was granule centralization, increased activated glycoprotein IIb-IIIa (GPIIb-IIIa) expression, increased platelet activation marker (PAC-1), increased fibrinogen binding, and decreased CD63 consistent with platelet activation. Intracellular serotonin (5-HT) levels were twice as high after incubation of PRP at 20 degrees C in the presence of serotonin re-uptake inhibitors compared to incubation at 37 degrees C.

   Biospecimens

   • Bodily Fluid - Plasma
   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Morphology	Light microscopy
   Morphology	Electron microscopy
   Protein	Flow cytometry
   Small molecule	Immunohistochemistry
   Small molecule	HPLC
   Small molecule	ELISA

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage temperature	20 degrees C 22 degrees C 24 degrees C 26 degrees C 28 degrees C 30 degrees C 34 degrees C 37 degrees C 42 degrees C
   Biospecimen Acquisition	Anticoagulant	Acid-citrate-dextrose Sodium citrate
   Storage	Storage duration	10 min 30 min 60 min 3 h 6 h
   Flow cytometry Specific	Targeted peptide/protein	Activated glycoprotein IIb-IIIa CD42b Fibrinogen CD41 CD62P CD63
   ELISA Specific	Targeted peptide/protein	Serotonin

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