Stability of intact parathyroid hormone in samples from hemodialysis patients.

Author(s): Cavalier E, Delanaye P, Carlisi A, Krzesinski JM, Chapelle JP

Publication: Kidney Int, 2007, Vol. 72, Page 370-2

PubMed ID: 17554256 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of storage on PTH levels in EDTA-plasma and serum using the Roche Elecsys assay.

   Summary of Findings:

   There was no difference in PTH levels between serum and EDTA-plasma with no storage or when specimens were stored at -20 degrees C for up to 24 h, but after 5 days at -20 degrees C, PTH levels in EDTA-plasma were decreased by 10.8% and serum levels were decreased by 7.2% (p<0.0004). In contrast, when specimens were stored at room temperature for 24 h, EDTA-plasma PTH levels only decreased by 4.1%, but serum levels declined by 20.5%. The authors report that the decrease in serum PTH levels after storage at room temperature would result in misclassification of 33% of patients. The authors conclude that if rapid delivery to the laboratory is not possible, specimens should be collected in EDTA tubes and analyzed within 24 h, but that EDTA-plasma should not be frozen.

   Biospecimens

   • Bodily Fluid - Serum
   • Bodily Fluid - Plasma

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Other diagnoses

   Platform:

   Analyte	Technology Platform
   Peptide	Clinical chemistry/auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Aliquots and Components	Blood and blood products	Plasma Serum
   Storage	Storage temperature	Room temperature -20 degrees C
   Storage	Storage duration	0 h 8 h 24 h 5 days
   Preaquisition	Diagnosis/ patient condition	Hemodialysis patients
   Biospecimen Acquisition	Time of biospecimen collection	October December

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