Stability of HIV-1 RNA in blood during specimen handling and storage prior to amplification by NASBA-QT.
Author(s): Bruisten SM, Oudshoorn P, van Swieten P, Boeser-Nunnink B, van Aarle P, Tondreau SP, Cuypers HT
Publication: J Virol Methods, 1997, Vol. 67, Page 199-207
PubMed ID: 9300385 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to determine the effects of anticoagulant and storage on the amplification of HIV-1 RNA using the NASBA-QT assay. After experimental storage of whole blood, lysis buffer was added to plasma and specimens were stored at -70 degrees C until analysis.
Summary of Findings:
The HIV-1 load was significantly lower in heparinized plasma or serum than in EDTA-plasma (p<0.0001, both). When EDTA-whole blood was stored at 4 degrees for 6-24 h, the HIV-1 RNA levels decreased slightly compared to levels in EDTA-plasma separated immediately, but storage of EDTA-whole blood at 25 degrees C for up to 72 h had no effects on HIV-1 RNA levels. Storage of heparinized whole blood at 4 or 25 degrees C, led to decreased HIV-1 RNA levels starting at 6 h. HIV-1 levels in EDTA-plasma and serum with lysis buffer were stable during 6 months of storage at -70 degrees C, and 14 days of storage at 4 degrees C, but the HIV-1 loads in serum and EDTA-plasma stored with lysis buffer at 30 degrees C were stable for only 1 day.
Biospecimens
Preservative Types
- Frozen
Diagnoses:
- AIDS/HIV-related
Platform:
Analyte Technology Platform RNA Nucleic acid sequence-based amplification (NASBA) Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Storage temperature -70 degrees C
4 degrees C
25 degrees C
30 degrees C
Storage Storage duration 0 h
6 h
24 h
48 h
72 h
7 days
14 days
1 month
3 months
6 months
Biospecimen Aliquots and Components Blood and blood products Plasma
Serum
Whole blood
Biospecimen Acquisition Anticoagulant EDTA
Heparin
Biospecimen Aliquots and Components Centrifugation Centrifugation delays investigated