Effects of low temperature on shear-induced platelet aggregation and activation.

Author(s): Zhang JN, Wood J, Bergeron AL, McBride L, Ball C, Yu Q, Pusiteri AE, Holcomb JB, Dong JF

Publication: J Trauma, 2004, Vol. 57, Page 216-23

PubMed ID: 15345964 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine if physiologically relevant hypothermic temperatures (32-37 degrees C) induce platelet activation or aggregation in whole blood or platelet-rich plasma.

   Summary of Findings:

   Platelet aggregation was significantly greater in both whole blood and platelet-rich plasma at 24, 32, and 35 degrees C compared to 37 degrees in response to fluid sheer stress induction, although a more robust induction was observed with whole blood. Platelet activation was not induced at physiologically relevant hypothermic temperatures; however, low temperatures did increase blood viscosity and plasma protein levels.

   Biospecimens

   • Bodily Fluid - Blood
   • Bodily Fluid - Plasma

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Cell count/volume	Coulter counter
   Cell count/volume	Flow cytometry
   Glycoprotein	Flow cytometry

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage temperature	24 degrees C 32 degrees C 35 degrees C 37 degrees C
   Biospecimen Aliquots and Components	Blood and blood products	Platelet-rich plasma Whole blood

   View more