The effect of time between venipuncture, processing and freezing on the measurement of coagulation factor levels.

Author(s): Engbers MJ, Cushman M, Rosendaal FR, Van Hylckama Vlieg A

Publication: J Thromb Haemost, 2012, Vol. 10, Page 1691-3

PubMed ID: 22632130 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of storing blood for 2.5 h at room temperature or on ice, prior to centrifugation, on measured levels of coagulation factors. Sodium citrate blood was collected from the first venipuncture from 10 patients with a maximum of 25 s tourniquet time. After centrifugation plasma was stored at -80 degrees C until analysis.

   Summary of Findings:

   None of the analytes were affected by a 2.5 h room temperature delay in centrifugation, but fibrinogen levels increased and factor VIII levels were decreased when specimens were stored on ice for 2.5 h prior to centrifugation compared to those centrifuged within the hour. Levels of prothrombin, antithrombin, FVIII, FIX, and FXI were unaffected by delaying centrifugation by 2.5 h, regardless of temperature. The authors report that the changes were similar for individuals with high and low coagulation factor levels.

   Biospecimens

   • Bodily Fluid - Blood
   • Bodily Fluid - Plasma

   Preservative Types

   • Frozen

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Glycoprotein	Hematology/ auto analyzer
   Protein	Hematology/ auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage temperature	Room temperature (21 degrees C) On ice (~4 degrees C)
   Storage	Storage duration	0 h 2.5 h
   Preaquisition	Biomarker level	High levels coagulation factors Low levels of coagulation factors
   Biospecimen Aliquots and Components	Centrifugation	Centrifugation delays investigated

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