Thrombin generation is extremely sensitive to preheating conditions.
Author(s): De Smedt E, Hemker HC
Publication: J Thromb Haemost, 2011, Vol. 9, Page 233-4
PubMed ID: 21062415 PubMed Review Paper? No
Purpose of Paper
The purpose of this paper was to investigate the effects of preheating time on thrombin generation in plasma.
Conclusion of Paper
The endogenous thrombin potential was overestimated by 50% when specimens were preheated in the instrument for 2 min. Preheating in the instrument for more than 2 minutes resulted in specimen evaporation and up to a 4 degree C difference in temperature between wells. When specimens were preheated for 5-15 min on the plate heater, thrombin generation became consistent.
Studies
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Study Purpose
The purpose of this study was to investigate the effects of preheating time on thrombin generation in plasma.
Summary of Findings:
The endogenous thrombin potential was overestimated by 50% when specimens were preheated in the instrument for 2 min. Preheating in the instrument for more than 2 minutes resulted in specimen evaporation and up to a 4 degree C difference in temperature between wells. When specimens were preheated for 5-15 min on the plate heater, thrombin generation became consistent.
Biospecimens
Preservative Types
- None (Fresh)
Diagnoses:
- Normal
Platform:
Analyte Technology Platform Protein Hematology/ auto analyzer Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Preaquisition Biomarker level 5 pM tissue factor
0.5 pM tissue factor
Hematology/ auto analyzer Specific Incubation temperature 37 degrees C
Hematology/ auto analyzer Specific Incubation time 2 min
5 min
10 min
15 min
Hematology/ auto analyzer Specific Heating method Covered on plate-heater
In fluorometer