The Relationship Between Cell-Free DNA and Resistance Training Volume Load.
Author(s): Lang HM, Duffourc MM, Bazyler CD, Ramsey MW, Gentles JA
Publication: J Strength Cond Res, 2024, Vol. 38, Page 1008-1012
PubMed ID: 38373069 PubMed Review Paper? No
Purpose of Paper
This paper compared cell-free DNA (cfDNA) levels in plasma obtained from healthy volunteers before and immediately following 3 or 6 sets of back squats and investigated if the relative strength of a participant was predictive of an increase in cfDNA levels following resistance exercise.
Conclusion of Paper
cfDNA levels were higher than levels taken at baseline (prior to exercise) after 3 or 6 sets of back squats, but cfDNA levels did not differ between plasma obtained after 3 and 6 sets. The authors developed a linear regression model that could predict the increase in cfDNA levels after 6 sets of back squats based on the participant's relative strength.
Studies
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Study Purpose
This study compared cfDNA levels in plasma obtained from healthy volunteers before and immediately following 3 or 6 sets of back squats and investigated if the relative strength of a participant was predictive of an increase in cfDNA levels following resistance exercise. Blood was collected into Vacutainer tubes from 15 healthy men and 15 healthy women immediately before (baseline) and after 3 sets (10 repetitions/set) and 6 sets of back squat exercise. Within 30 min of venipuncture, plasma was separated from blood by centrifugation at 6,000 rpm for 15 minutes. Plasma was stored in at -86°C until cfDNA isolation using the QIAamp MinElute ccfDNA Midi Kit. cfDNA was quantified by real-time PCR amplification of the RNase-P gene. The relative strength of each volunteer was calculated as the single repetition maximum divided by the weight of the volunteer.
Summary of Findings:
Compared to plasma collected immediately prior to squats (baseline), cfDNA concentrations were approximately 3-fold higher in plasma obtained after 3 sets (1,244.6 ± 875.83 versus 407.72 ± 320.83 ng/µL, p<0.01) or 6 sets (1,331.15 ± 1,141.66 versus 407.72 ± 320.83 ng/µL, p<0.01) of back squats, but cfDNA concentration did not differ between plasma obtained after 3 and 6 sets. The linear regression model was able to predict the change in cfDNA concentration from baseline after 6 sets of squats based on a participant’s relative strength (R2 =0.15, P= 0.04), but it was not significantly predictive after 3 sets of squats (R2 =0.12, P= 0.06).
Biospecimens
Preservative Types
- Frozen
Diagnoses:
- Normal
Platform:
Analyte Technology Platform DNA Real-time qPCR Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Preaquisition Diagnosis/ patient condition Relative strength calculated and compared
Biospecimen Acquisition Time of biospecimen collection Before exercise
After 3 sets of back squats
After 6 sets of back squats