The stability of the circulating human proteome to variations in sample collection and handling procedures measured with an aptamer-based proteomics array.
Author(s): Ostroff R, Foreman T, Keeney TR, Stratford S, Walker JJ, Zichi D
Publication: J Proteomics, 2010, Vol. 73, Page 649-66
PubMed ID: 19755178 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to determine the effects of collection tube type, delayed centrifugation, and delayed freezing on protein concentrations measured by an aptamer-based proteomics array in serum and plasma. Specimens were stored for various durations at room temperature either before centrifugation or between centrifugation and freezing. For the 20 h duration, however, storage prior to centrifugation was for 4 h at room temperature followed by 16 h at 4 degrees C, and storage between centrifugation and freezing was for 6 h at room temperature followed by 14 h at 4 degrees C.
Summary of Findings:
Of 498 proteins investigated, 83% were stable, regardless of collection tube type, storage for 30 min-20 h between blood collection and centrifugation or between centrifugation and freezing, or analysis of plasma or serum. In plasma, 90% of proteins were stable across all storage conditions. In Red Top (no additive) and SST serum specimens, 86% and 73% of proteins were stable across all storage conditions. Nearly all of the instability in serum or plasma specimens resulted when specimens were stored at room temperature for more than 2 h either before centrifugation or between centrifugation and freezing.
Biospecimens
Preservative Types
- Frozen
Diagnoses:
- Not specified
Platform:
Analyte Technology Platform Protein Aptamer-based microarray Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Aliquots and Components Blood and blood products Plasma
Serum
Biospecimen Acquisition Type of collection container/solution PPT EDTA plasma tube
SST serum separator tube
Red Top serum tube
Storage Time at room temperature 30 min
1 h
2 h
6 h
20 h
Biospecimen Aliquots and Components Centrifugation Centrifugation delays investigated