The stability of the circulating human proteome to variations in sample collection and handling procedures measured with an aptamer-based proteomics array.

Author(s): Ostroff R, Foreman T, Keeney TR, Stratford S, Walker JJ, Zichi D

Publication: J Proteomics, 2010, Vol. 73, Page 649-66

PubMed ID: 19755178 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of collection tube type, delayed centrifugation, and delayed freezing on protein concentrations measured by an aptamer-based proteomics array in serum and plasma. Specimens were stored for various durations at room temperature either before centrifugation or between centrifugation and freezing. For the 20 h duration, however, storage prior to centrifugation was for 4 h at room temperature followed by 16 h at 4 degrees C, and storage between centrifugation and freezing was for 6 h at room temperature followed by 14 h at 4 degrees C.

   Summary of Findings:

   Of 498 proteins investigated, 83% were stable, regardless of collection tube type, storage for 30 min-20 h between blood collection and centrifugation or between centrifugation and freezing, or analysis of plasma or serum. In plasma, 90% of proteins were stable across all storage conditions. In Red Top (no additive) and SST serum specimens, 86% and 73% of proteins were stable across all storage conditions. Nearly all of the instability in serum or plasma specimens resulted when specimens were stored at room temperature for more than 2 h either before centrifugation or between centrifugation and freezing.

   Biospecimens

   • Bodily Fluid - Plasma
   • Bodily Fluid - Serum
   • Bodily Fluid - Blood

   Preservative Types

   • Frozen

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Protein	Aptamer-based microarray

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Aliquots and Components	Blood and blood products	Plasma Serum
   Biospecimen Acquisition	Type of collection container/solution	PPT EDTA plasma tube SST serum separator tube Red Top serum tube
   Storage	Time at room temperature	30 min 1 h 2 h 6 h 20 h
   Biospecimen Aliquots and Components	Centrifugation	Centrifugation delays investigated

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