Cancer Diagnosis Program | Biorepositories and Biospecimen Research Branch

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Accuracy of clean-catch urine collection in infancy.

Author(s): Ramage IJ, Chapman JP, Hollman AS, Elabassi M, McColl JH, Beattie TJ

Publication: J Pediatr, 1999, Vol. 135, Page 765-7

PubMed ID: 10586183 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   This study compared the culture results obtained in CCU with those obtained using SPA specimens from infants suspected of having a UTI and investigated if ultrasound increased the SPA success. Both SPA and CCU specimens were collected from 49 patients (57 paired specimens) within 48 h of each other without use of antibiotics prior to collection or in the intervening time. To collect the CCU, infants were held on the lap (cleaned first if soiled) with a sterile foil bowl appropriately placed. SPA was obtained after ultrasound to ensure adequate urine in 28 cases. The SPA specimen was collected first in 28 pairs with a mean time between SPA and clean-catch of 3.6 h. The SPA and CCU were collected simultaneously in 18 cases. Urine was inoculated onto dip-slides and cultured for at least 16 h before analysis. A positive culture was defined as more than 10⁵ cfu/mL of a single organism in a CCU specimen or any growth in a SPA specimen.

   Summary of Findings:

   CCU had a sensitivity of 88.9%, a specificity of 95.0%, a false positive rate of 5%, and a false negative rate of 12% for positive results obtained by SPA. The majority of paired positive specimens (14 of 16) grew Escherichia coli with Pseudomonas aeruginosa and Candida albicans each cultured in one pair. Of the two false negative specimens, one grew Escherichia coli in the SPA and 10³ cfu/mL Escherichia coli in the CCU and the other grew Escherichia coli in the SPA and a mixed growth in the CCU. Performing ultrasound prior to SPA increased SPA success on the first attempt from 13 of 21 patients to 26 of 28 patients (62% to 93%, P=0.008).

   Biospecimens

   • Bodily Fluid - Urine

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Other diagnoses

   Platform:

   Analyte	Technology Platform
   Cell count/volume	Microbiological assay

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Method of fluid acquisition	Voided urine (24-h collection) Needle Different urine collection procedures compared
   Preaquisition	Non-invasive procedure type	Ultrasound None

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