The stability of the oxidative stress marker, urinary 8-hydroxy-2'- deoxyguanosine (8-OHdG), when stored at room temperature.

Author(s): Matsumoto Y, Ogawa Y, Yoshida R, Shimamori A, Kasai H, Ohta H

Publication: J Occup Health, 2008, Vol. 50, Page 366-72

PubMed ID: 18560203 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of storage temperature and duration on the stability of 8-OHdG in urine. After initial experimental storage conditions, all specimens were frozen at -80 degrees C until analysis.

   Summary of Findings:

   8-OHdG levels were unaffected by storage at 4 or 25 degrees C for 24 h when compared to levels measured in urine that was stored immediately at -80 degrees C prior to analysis. Additionally, no significant differences were seen in 8-OHdG levels in urine stored at -80 degrees C for around 200 days compared to specimens stored at -80 degrees C for around 1000 days. While patient age and gender had no effects on 8-OHdG concentration in urine, 8-OHdG levels in urine from smokers tended to be higher after storage at 25 degrees C compared to levels after storage at 4 degrees C, and 8-OHdG levels in urine from habitual medicine users tended to be lower after storage at 25 degrees C compared to levels after storage at 4 degrees C.

   Biospecimens

   • Bodily Fluid - Urine

   Preservative Types

   • Frozen

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Small molecule	HPLC

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage temperature	-80 degrees C 4 degrees C 25 degrees C
   Storage	Storage duration	0 h 24 h 194-197 d 1000 d
   Preaquisition	Patient age	23-58 y
   Preaquisition	Other drugs	Smoker Non-smoker Habitual medicine user No habitual use of medicine
   Preaquisition	Patient gender	Female Male

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