Stability of vitamin B-6-dependent aminotransferase activity in frozen packed erythrocytes is dependent on storage temperature.

Author(s): Hansen CM, Shultz TD

Publication: J Nutr, 2001, Vol. 131, Page 1581-3

PubMed ID: 11340119 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effect of storage temperature (-20, -80, or -196 degrees C) and duration (0-84 days) on basal and PLP stimulated EAST and EALT activities.

   Summary of Findings:

   After 58 days of storage at -20 degrees C, basal levels of EALT dropped 17% from the day 0 values, and EAST levels declined by 40% (p<0.05). No change in the basal levels of EALT or EAST were observed in specimens stored at -80 or -196 degrees C. The variability of the basal EAST levels was 2 times that of the basal EALT levels. The EALT-AC was not impacted by storage temperature or duration, but a nonsignificant trend toward declining levels with storage longer than 28 days was observed. Similarly, the EAST-AC did not differ significantly from day 0 at any timepoint, however differences between the storage temperatures were observed. In conclusion, for evaluating vitamin B6 status EALT-AC should be used and specimens for basal analysis should not be stored for more than 28 days at -20 degrees C but can be stored for at least 84 days at -80 or -196 degrees C.

   Biospecimens

   • Cell - Red Blood Cells

   Preservative Types

   • Frozen

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Protein	Spectrophotometry

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Aliquots and Components	Blood and blood products	Red blood cells
   Storage	Storage temperature	-20 degrees C -80 degrees C -196 degrees C
   Storage	Storage duration	1 day 7 days 14 days 28 days 58 days 84 days 0 days

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