Cancer Diagnosis Program | Biorepositories and Biospecimen Research Branch

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Stability of RNA transcripts in post-mortem psychiatric brains.

Author(s): Schramm M, Falkai P, Tepest R, Schneider-Axmann T, Przkora R, Waha A, Pietsch T, Bonte W, Bayer TA

Publication: J Neural Transm, 1999, Vol. 106, Page 329-35

PubMed ID: 10392541 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine if RT-PCR analysis of brain specimens is adversely impacted by the duration of postmortem interval (PMI). Twelve brain specimens were used for analysis and the temperature of PMI was not specified.

   Summary of Findings:

   Relative amounts of Trk B and Trk C, which were normalized to genes the authors identified as constitutive housekeeping genes, were variable among specimens and were not correlated to postmortem interval, gender, or disease-state.

   Biospecimens

   • Tissue - Brain

   Preservative Types

   • Frozen

   Diagnoses:

   • Normal
   • Schizophrenia
   • Other diagnoses

   Platform:

   Analyte	Technology Platform
   RNA	Real-time qRT-PCR

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Preaquisition	Patient gender	Female Male
   Preaquisition	Postmortem interval	1 d 2 d 3 d 4 d
   Preaquisition	Diagnosis/ patient condition	Normal Schizophrenia Alcoholism
   Real-time qRT-PCR Specific	Targeted nucleic acid	Trk B Trk C

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