Storage at -80 degrees C decreases the concentration of HPLC-detected urinary albumin: possible mechanisms and implications.

Author(s): Markó L, Cseh J, Kószegi T, Szabó Z, Molnár GA, Mohás M, Szigeti N, Wittmann I

Publication: J Nephrol, 2009, Vol. 22, Page 397-402

PubMed ID: 19557717 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of storage of urine specimens at -80 degrees C on HPLC-detected UAC.

   Summary of Findings:

   Storage of urine at -80 degrees C for 2.5 years resulted in decreased UAC by an average of 24%. Lower urinary pH was associated with a greater decrease in HPLC-detected UAC. The DMR of urinary albumin increased in stored specimens with significant changes in the peak area of the monomeric but not dimeric form. TFSG were significantly lower in stored urine specimens and did not correlate with specimen pH. On the other hand, in fresh specimens, an exponential correlation with urine pH was observed.

   Biospecimens

   • Bodily Fluid - Urine

   Preservative Types

   • Frozen

   Diagnoses:

   • Diabetes Type 2
   • Other diagnoses

   Platform:

   Analyte	Technology Platform
   Protein	HPLC
   Electrolyte/Metal	pH
   Small molecule	Spectrophotometry

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage temperature	-80 degrees C
   Preaquisition	Diagnosis/ patient condition	Normoalbuminuria Microalbuminuria
   Storage	Storage duration	Less than 2 weeks 2.5 y
   Biospecimen Aliquots and Components	pH	High pH Low pH

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