Re: loss of tumor marker-immunostaining intensity on stored paraffin slides of breast cancer.
Author(s): Manne U, Myers RB, Srivastava S, Grizzle WE
Publication: J Natl Cancer Inst, 1997, Vol. 89, Page 585-6
PubMed ID: 9106651 PubMed Review Paper? No
Purpose of Paper
This paper examined p53 and Bcl-2 antigen stability in (formalin-fixed) tissue sections freshly cut from archived paraffin blocks.
Conclusion of Paper
Both p53 and Bcl-2 staining intensity and the number of positive cases did not differ among paraffin blocks stored for 3-16 y or 7-12 y, respectively, when freshly cut tissue sections were used for analysis.
Studies
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Study Purpose
The purpose of this study was to determine if antigen stability (p53 and Bcl-2) remains adequately preserved after prolonged storage of formalin-fixed paraffin blocks. Of note, slides were prepared within 3 d of analysis.
Summary of Findings:
Both p53 and Bcl-2 staining intensity and the number of positive cases did not differ among paraffin blocks stored for 3-16 y or 7-12 y, respectively, when freshly cut tissue sections were used for analysis.
Biospecimens
Preservative Types
- Formalin
Diagnoses:
- Neoplastic - Carcinoma
Platform:
Analyte Technology Platform Protein Immunohistochemistry Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Storage duration 3-16 y
7-12 y
Immunohistochemistry Specific Targeted peptide/protein p53
Bcl-2