Cancer Diagnosis Program | Biorepositories and Biospecimen Research Branch

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Effects of Prolonged Storage of Whole Plasma or Isolated Plasma DNA on the Results of Circulating DNA Quantification Assays

Author(s): Sozzi G, Roz L, Conte D, Mariani L, Andriani F, Verderio P, Pastorino U

Publication: J Nat Cancer Inst, 2005, Vol. 97, Page 1848

PubMed ID: 16368947 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   To determine the impact of long-term storage of plasma at -80 degrees C and purified plasma DNA at -20 degrees C on DNA quantification of the human telomerase reverse transcriptase (hTERT) gene by real time quantitative PCR.

   Summary of Findings:

   DNA content significantly decreased as a function of storage duration in both plasma aliquots and purified plasma DNA by approximately 30% per year. No significant differences were observed between normal and cancerous specimens.

   Biospecimens

   • Bodily Fluid - Plasma

   Preservative Types

   • Frozen

   Diagnoses:

   • Neoplastic - Carcinoma
   • Normal

   Platform:

   Analyte	Technology Platform
   DNA	Real-time qPCR

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage duration	4 months 56 months
   Storage	Storage temperature	-20 degrees C -80 degrees C
   Real-time qPCR Specific	Targeted nucleic acid	hTERT

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