Effects of temperature and time delay on arterial blood gas and electrolyte measurements.

Author(s): Srisan P, Udomsri T, Jetanachai P, Lochindarat S, Kanjanapattanakul W

Publication: J Med Assoc Thai, 2011, Vol. 94 Suppl 3, Page S9-14

PubMed ID: 22043748 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of storage before analysis on arterial blood gas and electrolyte levels in pediatric blood specimens. Blood was collected from the arterial line of pediatric patients, with stable hemodynamic status, into heparinized capillary tubes, and stored until analysis. The authors use a p-value of <0.001 as the cut-off for significance.

   Summary of Findings:

   pH declined with storage of capillary blood specimens at room temperature or on ice, with significant differences from baseline first observed at 30 min and 45 min, respectively (p<0.001, both). The PO2 increased, and the PCO2 significantly decreased from baseline after only 15 min, regardless of storage temperature, but the changes were larger in specimens stored at room temperature than on ice. Chloride levels were higher after only 15 min at room temperature or 30 min on ice than at baseline, but sodium and potassium levels were not significantly affected by storage on ice. Further, only after 60 min of storage at room temperature were sodium levels lower and potassium levels higher than baseline (p<0.001).

   Biospecimens

   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Gas	Clinical chemistry/auto analyzer
   Small molecule	Clinical chemistry/auto analyzer
   Electrolyte/Metal	Clinical chemistry/auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)

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