Quantitation of isoprostane isomers in human urine from smokers and nonsmokers by LC-MS/MS.
Author(s): Yan W, Byrd GD, Ogden MW
Publication: J Lipid Res, 2007, Vol. 48, Page 1607-17
PubMed ID: 17456897 PubMed Review Paper? No
Purpose of Paper
This paper investigated the effects of cigarette smoking and gender on measurement of the isoprostanes and prostaglandins in urine.
Conclusion of Paper
Urine from smokers contained higher creatinine-normalized levels of iPF2a-III and 2,3-Dinor-iPF2α-III than urine from non-smokers and a higher daily level of iPF2a-III. Urinary levels of 8,12-Iso-iPF2α-VI and iPF2α-VI were strongly correlated and each was also modestly correlated with nicotine uptake. Females had significantly higher creatinine-normalized levels of iPF2α-III, 2,3-dinor-iPF2α-III, 15- epi-iPF2α-III, 8,12-iso-iPF2α-VI, and iPF2α-VI than males, but this difference was not significant when normalized by day.
Studies
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Study Purpose
This study investigated the effects of cigarette smoking and gender on measurement of the isoprostanes and prostaglandins in urine. Two consecutive 24 h urine specimens were collected from 32 smokers and 12 non-smokers a week after starting a low-mutagen diet. Creatinine was determined by ELISA and the other analytes by LC-MS/MS. Nicotine uptake was determined by adding the levels of nicotine and nicotine metabolites present in urine.
Summary of Findings:
The mean isoprostane iPF2α-III level was higher in the urine from smokers than non-smokers when measured by LC-MS/MS (513 versus 294 pg/mg creatinine, P=0.0015) or ELISA (1152 versus 604 pg/mg creatinine, P=0.0000). The mean 2,3-Dinor-iPF2α-III level was also higher in smokers than non-smokers (3030 versus 2046 pg/mg creatinine, P=0.0023). When values were adjusted to ug/day, only the differences in iPF2a-III were significant. The effects of smoking were observed in both males and females. Females had significantly higher creatinine-normalized levels of iPF2α-III, 2,3-dinor-iPF2α-III, 15- epi-iPF2α-III, 8,12-iso-iPF2α-VI, and iPF2α-VI than males, but this difference was not significant when levels per day were examined. Levels of 15-Epi-iPF2a-III, 8,12-Iso-iPF2α-VI, PGF2α, and iPF2α-VI were unaffected by smoking status. Importantly, excretion of 8,12-Iso-iPF2α-VI and iPF2α-VI were significantly correlated with nicotine uptake (r=0.4010, P= 0.0229 and r= 0.3723, P= 0.0359, respectively). As expected, levels of 8,12-Iso-iPF2α-VI and iPF2α-VI were significantly correlated (r=0.7619, P<0.0001).
Biospecimens
Preservative Types
- None (Fresh)
Diagnoses:
- Not specified
Platform:
Analyte Technology Platform Small molecule ELISA Small molecule LC-MS or LC-MS/MS Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Preaquisition Other drugs Smoker
Non-smoker
Preaquisition Patient gender Female
Male