Comparison of capillary and venous blood sampling for quantitation of plasma cholesterol.

Author(s): Ishikawa TT, Morrison J, Fallat R, Parsons D, Glueck CJ

Publication: J Lab Clin Med, 1974, Vol. 84, Page 281-6

PubMed ID: 4843232 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of blood collection method and method of analysis on cholesterol levels in 181 children and adults. Blood specimens were refrigerated at 4 degrees C for an unspecified interval prior to analysis.

   Summary of Findings:

   The mean cholesterol level of 34 patients was the same whether measured by GLC in venous or capillary blood or by the colorimetric assay in venous blood. The correlation between cholesterol levels in capillary blood measured by GLC and in venous blood measured using a colorimetric assay was r=0.96. Fasting plasma triglyceride levels in serum and finger squeezing during capillary blood collection did not affect the cholesterol levels. Finally, there was no significant difference in consistency between the two methods.

   Biospecimens

   • Bodily Fluid - Blood

   Preservative Types

   • Other Preservative

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Steroid	Colorimetric assay
   Steroid	GC

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Method of fluid acquisition	Venipuncture Finger/heel prick with squeezing Finger/heel prick sampling
   Preaquisition	Biomarker level	Fasting plasma triglycerides over 200 mg Fasting plasma triglycerides below 200 mg
   GC Specific	Technology platform	Colorimetric assay
   Biospecimen Acquisition	Anatomical location of blood draw	Capillary Vein

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