Apoptosis: a method for evaluating the cryopreservation of whole blood and peripheral blood mononuclear cells.
Author(s): Fowke KR, Behnke J, Hanson C, Shea K, Cosentino LM
Publication: J Immunol Methods, 2000, Vol. 244, Page 139-44
PubMed ID: 11033026 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
The purpose of this study was to determine the effects of storage at -30, -70, and -150 degrees C for 10 weeks on cell viability, levels of apoptosis, and the ability to be transformed by Epstein-Barr virus (EBV) of whole blood versus matched peripheral blood mononuclear cell (PBMC) specimens.
Summary of Findings:
A significant decrease in viability was detected in PBMC specimens stored at -30 degrees C compared to those stored at -70 or -150 degrees C (p<0.0001). Higher levels of spontaneous and activation-induced apoptosis were observed for both whole blood and PBMC specimens stored at -30 degrees C (p<0.001) than at -70 or -150 degrees C. The levels of spontaneous apoptosis in whole blood specimens at -70 or -150 degrees C were higher than those observed in matched PBMC specimens (p<0.005). EBV transformation of specimens stored at -30 degrees C was only possible in whole blood due to insufficient cell numbers from PBMC. In the whole blood specimens stored at -30 degrees C, transformation was only moderately successful in 1 of 4 specimens after 6 weeks. Specimens stored at lower temperatures were all able to be transformed in 2-4 weeks, however, the whole blood specimens appeared to initially lag behind the PBMC specimens with respect to transformation.
Analyte Technology Platform Morphology Fluorescent microscopy Morphology Light microscopy
Classification Pre-analytical Factor Value(s) Biospecimen Aliquots and Components Blood and blood products Peripheral blood mononuclear cells
Storage Storage temperature -30 degrees C
-70 degrees C
-150 degrees C
Storage Storage duration 10 d