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Influence of collection and separation of blood samples on plasma IL-1, IL-6 and TNF-alpha concentrations.

Author(s): Riches P, Gooding R, Millar BC, Rowbottom AW

Publication: J Immunol Methods, 1992, Vol. 153, Page 125-31

PubMed ID: 1517582 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of type of anticoagulant as well as storing blood at 37 degrees C before centrifugation on cytokine levels in plasma.

   Summary of Findings:

   Incubation of blood at 37 degrees C in lithium heparin tubes prior to centrifugation led to time dependent increases in IL-1 and IL-6 concentrations after 3 h and increases in TNF-alpha after 2 h. Pyrogen-free sodium heparin and EDTA did not have the same effects on cytokine concentration. When blood collected into lithium heparin tubes was transferred to EDTA tubes for the 37 degrees C storage prior to centrifugation, the EDTA inhibited cytokine production, as long as the transfer occurred immediately, and the inhibition of cytokine production decreased as the storage duration in the lithium heparin tube increased. Plasma collected in endotoxin-free lithium heparin tubes showed no IL-1 production when assayed by radioimmunoassay or bioassay. Further, incubation of blood in endotoxin-free lithium heparin tubes for 3 h prior to centrifugation did not cause any significant changes in IL-6 or IL-1 concentrations compared with plasma that was centrifuged immediately.

   Biospecimens

   • Bodily Fluid - Plasma
   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Protein	Radioimmunoassay
   Protein	ELISA
   Protein	Bioassay
   RNA	RT-PCR

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Anticoagulant	Lithium heparin Multiple forms evaluated EDTA Sodium heparin
   Storage	Storage duration	0 min 20 min 40 min 1 h 2 h 3 h 6 h
   Storage	Type of storage container	EDTA tube Lithium heparin tube Pyrogen-free sodium heparin tube
   Biospecimen Aliquots and Components	Centrifugation	Centrifugation delays investigated

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